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(Plasmid #58738)


Item Catalog # Description Quantity Price (USD)
Plasmid 58738 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 4731
  • Total vector size (bp) 9624
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • GenBank ID
  • Entrez Gene
    SCRIB (a.k.a. CRIB1, SCRB1, SCRIB1, Vartul, oSCRIB)
  • Promoter CMV
  • Tag / Fusion Protein
    • Venus (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site EcoR1 (not destroyed)
  • 5′ sequencing primer CMV forward
  • 3′ sequencing primer EGFP reverse
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A partial cDNA to human SCRIB was provided by Jon Huibregtse (Univ. of Texas, Austin TX). To generate a full length ORF, we amplified the 5' and 3' ends of the complete open reading frame by PCR from a human kidney cDNA library and ligated them into the partial clone.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKvenus-Scribble was a gift from Ian Macara (Addgene plasmid # 58738 ; ; RRID:Addgene_58738)
  • For your References section:

    The function of the p190 Rho GTPase-activating protein is controlled by its N-terminal GTP binding domain. Tatsis N, Lannigan DA, Macara IG. J Biol Chem. 1998 Dec 18. 273(51):34631-8. 10.1074/jbc.273.51.34631 PubMed 9852136