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pCAG-HA-Nrxn1beta AS4(-)
(Plasmid #59409)


Item Catalog # Description Quantity Price (USD)
Plasmid 59409 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 4869
  • Total vector size (bp) 6213
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    Nrxn1beta AS4(-)
  • Alt name
    Neurexin 1beta AS4(-)
  • Alt name
    Neurexin 1beta Delta4
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
  • Entrez Gene
    Nrxn1 (a.k.a. 1700062G21Rik, 9330127H16Rik, A230068P09Rik, mKIAA0578)
  • Promoter CAG
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer pCAG-F (5'-GCAACGTGCTGGTTATTGTG-3')
  • 3′ sequencing primer Bglob-pA-R (5'-TTTTGGCAGAGGGAAAAAGA-3'); pCAG_rev_seq (5'-CAGCCACCACCTTCTGATAG-3')
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See map & sequence file for insert-releasing enzymes (e.g. NheI / XmaI).
The Nrxn 1 beta variant encoded by this plasmid is AS4(-) / AS5(-):
- Exon 21 constituting alternatively spliced segment 4 (AS4) (=ENSMUSE00001309616) is absent.
- AS5 is absent, i.e. the short form of exon 23 (23.2) is present (=ENSMUSE00001290978; "GTGGGTTAG" missing compared to (longer) exon 23.1 (=ENSMUSE00000862714)).
See Schreiner et al., Neuron, 2014 for nomenclature of alternatively spliced segments (doi:10.1016/j.neuron.2014.09.011).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-HA-Nrxn1beta AS4(-) was a gift from Peter Scheiffele (Addgene plasmid # 59409 ; ; RRID:Addgene_59409)
  • For your References section:

    Alternative splicing controls selective trans-synaptic interactions of the neuroligin-neurexin complex. Chih B, Gollan L, Scheiffele P. Neuron. 2006 Jul 20. 51(2):171-8. 10.1016/j.neuron.2006.06.005 PubMed 16846852