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Addgene

pCruzHA SIRT1 G261A
(Plasmid #10963)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 10963 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCruz HA
  • Backbone manufacturer
    Santa Cruz Biotechnology
  • Backbone size w/o insert (bp) 6600
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SIRT1
  • Species
    M. musculus (mouse)
  • Mutation
    G261A
  • Entrez Gene
    Sirt1 (a.k.a. SIR2L1, Sir2, Sir2a, Sir2alpha)
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCruzHA SIRT1 G261A was a gift from Toren Finkel (Addgene plasmid # 10963 ; http://n2t.net/addgene:10963 ; RRID:Addgene_10963)
  • For your References section:

    SIRT1 functionally interacts with the metabolic regulator and transcriptional coactivator PGC-1{alpha}. Nemoto S, Fergusson MM, Finkel T. J Biol Chem. 2005 Apr 22. 280(16):16456-60. 10.1074/jbc.M501485200 PubMed 15716268