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Depositing Lab
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Depositing OrganizationEmory University
Located in United States of America -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 40872 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 40872-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 40872-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepcDNA-DEST47
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 7780
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameArl13b
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SpeciesM. musculus (mouse)
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Insert Size (bp)1410
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Mutationno stop codon
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Entrez GeneArl13b (a.k.a. A530097K21Rik, A930014M17Rik, Arl2l1, C530009C10Rik, hnn)
- Promoter CMV
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Tag
/ Fusion Protein
- GFP (C terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer T7 promoter
- 3′ sequencing primer BGH reverse
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
In Larkins et al (2011), this is plasmid "Arl13b GFP tagged" as referenced in Figure 2. Full-length Arl13b without a stop codon (pENTR-Arl13b2) was Gateway cloned into pcDNA-DEST47.
DNA (Catalog # 40872-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 40872-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDEST47-Arl13b12 was a gift from Tamara Caspary (Addgene plasmid # 40872 ; http://n2t.net/addgene:40872 ; RRID:Addgene_40872) -
For your References section:
Arl13b regulates ciliogenesis and the dynamic localization of Shh signaling proteins. Larkins CE, Aviles GD, East MP, Kahn RA, Caspary T. Mol Biol Cell. 2011 Dec;22(23):4694-703. Epub 2011 Oct 5. 10.1091/mbc.E10-12-0994 PubMed 21976698
Ready-to-use antibodies
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