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Purpose(Empty Backbone) a bidirectional reporter vector allowing the study of biscistronic promoter
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 64034 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepGL4.10
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Backbone manufacturerPromega
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Modifications to backboneAdded Rluc in Asp718/NheI sites
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Vector typeMammalian Expression, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGL4Luc-RLuc was a gift from Daniel Christophe (Addgene plasmid # 64034 ; http://n2t.net/addgene:64034 ; RRID:Addgene_64034) -
For your References section:
Delimitation and functional characterization of the bidirectional THOX-DUOXA promoter regions in thyrocytes. Christophe-Hobertus C, Christophe D. Mol Cell Endocrinol. 2010 Apr 12;317(1-2):161-7. doi: 10.1016/j.mce.2010.01.001. Epub 2010 Jan 12. 10.1016/j.mce.2010.01.001 PubMed 20060878
Map uploaded by the depositor.
