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Daniel Bauer Lab Plasmids


The Daniel Bauer Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

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Title Authors Publication
BCL11A enhancer dissection by Cas9-mediated in situ saturating mutagenesis. Canver MC, Smith EC, Sher F, Pinello L, Sanjana NE, Shalem O, Chen DD, Schupp PG, Vinjamur DS, Garcia SP, Luc S, Kurita R, Nakamura Y, Fujiwara Y, Maeda T, Yuan GC, Zhang F, Orkin SH, Bauer DE Nature. 2015 Sep 16. doi: 10.1038/nature15521.
Variant-aware saturating mutagenesis using multiple Cas9 nucleases identifies regulatory elements at trait-associated loci. Canver MC, Lessard S, Pinello L, Wu Y, Ilboudo Y, Stern EN, Needleman AJ, Galacteros F, Brugnara C, Kutlar A, McKenzie C, Reid M, Chen DD, Das PP, A Cole M, Zeng J, Kurita R, Nakamura Y, Yuan GC, Lettre G, Bauer DE, Orkin SH Nat Genet. 2017 Feb 20. doi: 10.1038/ng.3793.
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
70267lentiCas9-VenusCas9 (Synthetic), Venus Reporter (Synthetic)Mammalian Expression, Lentiviral, CRISPRBCL11A enhancer dissection by Cas9-mediated in situ saturating mutagenesis. Nature. 2015 Sep 16. doi: 10.1038/nature15521. Expresses human codon-optimized S. pyogenes Cas9 protein and Venus reporter from EFS promoter. Lentiviral backbone. pFUGW Add to Cart
70683lentiGuide-CrimsonS. pyogenes sgRNA cassette (Synthetic), Crimson Reporter (Synthetic)Mammalian Expression, Lentiviral, CRISPRBCL11A enhancer dissection by Cas9-mediated in situ saturating mutagenesis. Nature. 2015 Sep 16. doi: 10.1038/nature15521. Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and Crimson reporter from EF-1a promoter. Lentiviral backbone. Custom Add to Cart
87155lenti-Cas9-VQR-BlastSpCas9-VQR(D1135V,R1335Q,T1337R) (Other)LentiviralVariant-aware saturating mutagenesis using multiple Cas9 nucleases identifies regulatory elements at trait-associated loci. Nat Genet. 2017 Feb 20. doi: 10.1038/ng.3793. lentiviral expression of SpCas9-VQR (NGA PAM restricted) lentiCas9-Blast Add to Cart
87156lenti-Cas9-VQR-GFP_activity_reporterGFP and sgRNA targeting GFP (NGA restricted)LentiviralVariant-aware saturating mutagenesis using multiple Cas9 nucleases identifies regulatory elements at trait-associated loci. Nat Genet. 2017 Feb 20. doi: 10.1038/ng.3793. This lentiviral vector can be used to assay activity of SpCas9-VQR (NGA PAM restricted). pXPR_011 Add to Cart