160,922 results

1. MsACR1-TS-Kv-P2A-mCerulean3-pAAV-CaMKII

   Plasmid

   #204970

   Purpose

   Soma-targeted neuronal expression of MsACR1 and mCerulean3 separated by a P2A site.

   Depositor

   Peter Hegemann

   Article

   Oppermann et al eLife. 2024 Oct 14;12:RP90100. doi: 10.7

   Insert

   MsACR1
   

   Use

   AAV

   Tags

   mCerulean3

   Expression

   Mutation

   Promoter

   CaMKII

   Available since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBad-EBFP2

   Plasmid

   #14891

   Purpose

   Bacterial expression of EBFP2 blue fluorescent protein

   Depositor

   Robert Campbell

   Article

   Ai et al Biochemistry. 2007 May 22;46(20):5904-10

   Has Service

   Cloning Grade DNA

   Insert

   EBFP2
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   The fluorescent protein was inserted between XhoI…

   Promoter

   Available since

   May 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPB-tetO(hCMV1)-HA-Tet1mHxD(201R2)-IV

   Plasmid

   #102422

   Purpose

   Inducible expression of siRNA resistant mouse Tet1-201 (ENSMUST00000050826.13) with mutated catalytic domain (H1620Y & D1622A), HA-tag and IRES-Venus in piggyBac (PB) transposon vector

   Depositor

   Kian Peng Koh

   Article

   Khoueiry et al Nat Genet. 2017 Jul;49(7):1061-1072. doi

   Insert

   Tet1 catalytic domain mutant (Tet1 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   H1620Y and D1622A mutations in Tet1 catalytic dom…

   Promoter

   TetO-CMV

   Available since

   Nov. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHCKan-yibDp-mCherry

   Plasmid

   #127058

   Purpose

   Low phosphate inducible mCherry with N-terminal linker and His tag

   Depositor

   Michael Lynch

   Article

   Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   yibDp

   Available since

   May 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-CSF1R-V5/HIS

   Plasmid

   #201984

   Purpose

   expression of human CSF1R receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   CSF1R receptor tyrosine kinase (CSF1R Human)
   

   Use

   Tags

   V5, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJAM1.19_PB-CIT-PGK-Blast

   Plasmid

   #205564

   Purpose

   Express human CIT

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   CIT (CIT Human)
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   EF1

   Available since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLJM1 Raptor GFP

   Plasmid

   #112744

   Purpose

   express Raptor-GFP

   Depositor

   Roberto Zoncu

   Article

   Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

   Insert

   Raptor-GFP (RPTOR Human)
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mutation

   Promoter

   Available since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. ankG-mCherry

   Plasmid

   #42566

   Depositor

   Benedicte Dargent

   Article

   Leterrier et al Proc Natl Acad Sci U S A. 2011 May 24;10

   Insert

   ankyrin G (Ank3 Rat)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNR230

   Plasmid

   #79546

   Purpose

   BxbI attB-attP. GFP will express if sites recombine.

   Depositor

   Timothy Lu

   Article

   Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

   Insert

   GFPmut3b
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. SpCas9-CBE6d-V106W

    Plasmid

    #215827

    Purpose

    Express CBE6d V106W (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

    Insert

    CBE6d V106W-SpCas9-2xUGI (cas9 )
    

    Use

    In vitro transcription template

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. TFORF0629

    Plasmid

    #142667

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NR1I2 (NR1I2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFREE_Amp

    Plasmid

    #92051

    Purpose

    Allows inducible expression of gRNAs and Cas9 for plasmid curing.

    Depositor

    Morten Norholm

    Article

    Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

    Inserts

    Cas9
    gRNA array
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    pRhamBAD and ptet

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUASTattB-GFP-Msp300KASH

    Plasmid

    #170806

    Purpose

    Expresses Msp300 KASH domain fused to GFP under Gal4/UAS control for Drosophila transgenesis

    Depositor

    Vikki Weake

    Article

    Jauregui-Lozano et al Genetics. 2021 May 22. pii: 6281219. doi

    Insert

    GFP-Msp300KASH
    

    Use

    Tags

    GFP

    Expression

    Insect

    Mutation

    Promoter

    5xUAS + hsp70 minimal promoter

    Available since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDH-EF1-DIO-copGFP

    Plasmid

    #72253

    Purpose

    Express copGFP under EF-1 promoter when Cre is expressed by another vector

    Depositor

    Kazuhiro Oka

    Insert

    copGFP
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    elongation factor-1

    Available since

    Jan. 8, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pRN3P-HA-Suv4-20h1mut

    Plasmid

    #86690

    Purpose

    For transcription of of Suv4-20h1mut mRNA preceded by an HA-tag in 5'

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Eid et al Genes Dev. 2016 Nov 15;30(22):2513-2526.

    Insert

    histone-lysine N-methyltransferase KMT5B mutant (Kmt5b Mouse)
    

    Use

    Tags

    HA

    Expression

    Mutation

    mutated region NHDC (asparagine 273 to cysteine 2…

    Promoter

    T3

    Available since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJW1821

    Plasmid

    #163092

    Purpose

    CRISPR repair template: add insertion site specific homology arms by PCR before insertion

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    mScarlet^SEC (Lox511I)^::3xMyc
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNR276

    Plasmid

    #79577

    Purpose

    TP901 attB-attP. GFP will express if sites recombine.

    Depositor

    Timothy Lu

    Article

    Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

    Insert

    GFPmut3b
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pNR239

    Plasmid

    #79576

    Purpose

    A118 attB-attP. GFP will express if sites recombine.

    Depositor

    Timothy Lu

    Article

    Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

    Insert

    GFPmut3b
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV-hSyn-Axon-jGCaMP8s

    Plasmid

    #172720

    Purpose

    AAV transfer plasmid for neuronal expression of axon-targeted jGCaMP8s.

    Depositor

    Marianne Fyhn

    Article

    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

    Insert

    Axon-jGCaMP8s
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Dec. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. RGS-6xHis-BLIMP-1-pcDNA3.1-

    Plasmid

    #52518

    Purpose

    expresses human BLMP-1 in mammalian cells with RGS-6xHis tag at N-terminus

    Depositor

    Adam Antebi

    Article

    Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

    Insert

    Blimp1 (PRDM1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge