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V246 pCEP 4HA B56beta
(Plasmid #14533)


This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14533 Standard format: Plasmid sent in bacteria as agar stab 1 $85


  • Vector backbone
    pCEP4 (modified)
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 10186
  • Vector type
    Mammalian Expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    PPP2R5B B56 beta regulatory subunit of PP2A
  • Alt name
    B' beta
  • Alt name
    PR61 beta
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • GenBank ID
  • Entrez Gene
    PPP2R5B (a.k.a. B56B, B56beta, PR61B)
  • Tag / Fusion Protein
    • 4 HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer pCEP forward (invitrogen)
  • 3′ sequencing primer EBV Reverse (invitrogen)
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

4 HA tag cloned into Not1 site as Not1 cassette so two Not1 sites at 5' end of gene.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    V246 pCEP 4HA B56beta was a gift from David Virshup (Addgene plasmid # 14533 ; ; RRID:Addgene_14533)
  • For your References section:

    Regulation of beta-catenin signaling by the B56 subunit of protein phosphatase 2A. Seeling JM, Miller JR, Gil R, Moon RT, White R, Virshup DM. Science. 1999 Mar 26. 283(5410):2089-91. 10.1126/science.283.5410.2089 PubMed 10092233