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V245 pCEP-4HA B56alpha
(Plasmid #14532)


Item Catalog # Description Quantity Price (USD)
Plasmid 14532 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
    pCEP4 (modified)
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 10186
  • Vector type
    Mammalian Expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    PPP2R5A B56 alpha regulatory subunit of PP2A
  • Alt name
    B' alpha
  • Alt name
    PR61 alpha
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • GenBank ID
  • Entrez Gene
    PPP2R5A (a.k.a. B56A, B56alpha, PR61A)
  • Tag / Fusion Protein
    • 4 HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer pCEP forward (invitrogen)
  • 3′ sequencing primer EBV Reverse (invitrogen)
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

4 HA tag cloned into Not1 site as Not1 cassette so two Not1 sites at 5' end of gene.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    V245 pCEP-4HA B56alpha was a gift from David Virshup (Addgene plasmid # 14532 ; ; RRID:Addgene_14532)
  • For your References section:

    Regulation of beta-catenin signaling by the B56 subunit of protein phosphatase 2A. Seeling JM, Miller JR, Gil R, Moon RT, White R, Virshup DM. Science. 1999 Mar 26. 283(5410):2089-91. 10.1126/science.283.5410.2089 PubMed 10092233