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CRISPR/Cpf1 enables fast and simple genome editing of Saccharomyces cerevisiae.
Verwaal R, Buiting-Wiessenhaan N, Dalhuijsen S, Roubos JA
Yeast. 2017 Sep 8. doi: 10.1002/yea.3278.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
101725pCSN061Single copy yeast plasmid expressing Cas9 from Streptococcus pyogenes (SpCas9), codon optimized for expression in Saccharomyces cerevisiae.
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101748pCSN067Single copy yeast plasmid expressing Cpf1 from Lachnospiraceae bacterium ND2006 (LbCpf1), codon optimized for expression in Saccharomyces cerevisiae.
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101749pCSN068Single copy yeast plasmid expressing Cpf1 from Francisella novicida U112 (FnCpf1), codon optimized for expression in Saccharomyces cerevisiae.
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101750pRN1120Multi-copy (2 micron) yeast plasmid containing a functional NatMX marker cassette conferring resistance against nourseothricin. Recipient plasmid for sgRNA or crRNA expression cassettes.
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