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pCSN061
(Plasmid #101725)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 101725 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    S. pyogenes Cas9 codon optimized for expression in S. cerevisiae.
  • Alt name
    SpCas9
  • Insert Size (bp)
    4140
  • Entrez Gene
    NEWENTRY
  • Promoter Heterologous promoter from K. lactis (KLLA0F20031g)
  • Tag / Fusion Protein
    • SV40 NLS (C terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site KnpI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer .
  • 3′ sequencing primer .
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    KanMX marker expression cassette.
  • Promoter Heterologous TEF1 promoter from A. gossypii.

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer .
  • 3′ sequencing primer .
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCSN061 was a gift from Rene Verwaal (Addgene plasmid # 101725 ; http://n2t.net/addgene:101725 ; RRID:Addgene_101725)
  • For your References section:

    CRISPR/Cpf1 enables fast and simple genome editing of Saccharomyces cerevisiae. Verwaal R, Buiting-Wiessenhaan N, Dalhuijsen S, Roubos JA. Yeast. 2017 Sep 8. doi: 10.1002/yea.3278. 10.1002/yea.3278 PubMed 28886218