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YE1-BE4max

Plasmid

#138155

Purpose

C-to-T base editor

Depositor

David Liu

Article

Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

Insert

rAPOBEC1(YE1)-nSpCas9-UGI-UGI

Tags

NLS

Expression

Mammalian

Mutation

Within rAPOBEC1 W90Y + R126E; within Cas9 D10A

Promoter

CMV

Available Since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-cMSP1D1

Plasmid

#199768

Purpose

Expresses split-intein fused MSP1D1 for circularization during expression (external His-Tag)

Depositor

Franz Hagn

Article

Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

Insert

cMSP1D1

Tags

His6-Tag

Expression

Bacterial

Mutation

external His-Tags flanking the C and N termini

Available Since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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ZNF668-Flag

Plasmid

#42636

Depositor

Shiaw-Yih Lin

Article

Hu et al Cancer Res. 2011 Oct 15;71(20):6524-34.

Insert

ZNF668 (ZNF668 Human)

Tags

3xFLAG

Expression

Mammalian

Available Since

April 23, 2013

Availability

Academic Institutions and Nonprofits only
OLIG2_pCSdest

Plasmid

#53858

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

OLIG2 (OLIG2 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pC007 - pRFP constitutive expression

Plasmid

#79156

Purpose

RFP constitutively expressed

Depositor

Feng Zhang

Article

Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

Insert

RFP

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-endo-Nb37

Plasmid

#205514

Purpose

Endosome-targeted Nb37 nanobody that binds and stabilizes the active form of Gαs G-protein subunit.

Depositor

Jin Zhang

Article

Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

Insert

endo-Nb37

Tags

2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pOpen-MMLV_RT (mut H)

Plasmid

#165546

Purpose

Single 75 kDa monomer, cDNA synthesis; high enzyme activity and processivity.

Depositor

Drew Endy, Jennifer Molloy, FreeGenes Project

Insert

Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase (RNAse H deactivated by 3 mutations)

Use

Synthetic Biology

Available Since

Aug. 10, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pET29b-rhMOG

Plasmid

#225484

Purpose

Express the soluble domain of Myelin Oligodendrocyte Glycoprotein (rhMOG) in SHuffle E. coli

Depositor

John Pak

Article

Wu et al J Immunol Methods. 2024 Oct 13:113764. d

Insert

Soluble Domain of Myelin Oligodendrocyte Glycoprotein (MOG Human)

Tags

6xHis tag

Expression

Bacterial

Mutation

deleted amino acids 1-29 (signal peptide) and 150…

Promoter

T7

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST exorh:mCherry

Plasmid

#195989

Purpose

Transgenesis backbone

Depositor

Christian Mosimann, Nicolas Rohner

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Type

Empty backbone

Use

Other

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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CMV500 empty vector

Plasmid

#33348

Depositor

Charles Vinson

Article

Rishi et al J Biol Chem. 2004 Mar 19;279(12):11863-7

Type

Empty backbone

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2011

Availability

Academic Institutions and Nonprofits only

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pNvT-MHC::mCh

Plasmid

#67943

Purpose

Transgenesis vector consisting of a 1.6-kb fragment of MyHC1 promoter region upstream of the start codon and an mCherry reporter gene, flanked by inverted binding sites of meganuclease I-SceI

Depositor

Ulrich Technau

Article

Renfer et al Proc Natl Acad Sci U S A. 2010 Jan 5;107

Insert

mCherry

Use

Transgenic reporter in the sea anemone, nematoste…

Promoter

Myosin Heavy Chain1 (MyHC1)

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT TO-EGFP-G3BP1-WT

Plasmid

#136003

Purpose

DOX-inducible WT G3BP1 inserted with GFP tagged on the N-terminus and used with the Flp-In T-Rex System

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

G3BP1 (G3BP1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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PZac2.1 gfaABC1D-NLS-SaCas9-3xHA-NLS bGH

Plasmid

#178960

Purpose

Expresses saCas9 protein specifically in astrocytes

Depositor

Baljit Khakh

Article

Endo et al Science. 2022 Nov 4;378(6619):eadc9020.

Insert

Staphylococcus aureus Cas9 (SaCas9)

Use

AAV

Tags

3x HA

Promoter

gfaABC1D (astrocyte specific)

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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FUW-TetON-GFP

Plasmid

#115495

Purpose

Lentiviral plasmid for tet-inducible expression of GFP (generated from plasmid #20321)

Depositor

Catherine Verfaillie

Article

Garcia-Leon et al Stem Cell Reports. 2018 Feb 13;10(2):655

Insert

eGFP

Use

Lentiviral

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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dCas-hHDAC3-R265P

Plasmid

#103786

Purpose

Expresses dCas9 fused to human HDAC3 with R265P mutation

Depositor

Zhaolan Zhou

Article

Kwon et al Nat Commun. 2017 May 12;8:15315. doi: 10

Insert

dCas9-HDAC3-R265P (HDAC3 S. pyogenes, Synthetic, Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

Changed arginine 265 to proline

Promoter

EF1A

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pLJC5-3XMyc-EGFP-PEX26

Plasmid

#139059

Purpose

Myc-PEROXO Lentiviral Construct. Expresses a Peroxisomal Tag.

Depositor

David Sabatini

Article

Ray et al iScience. 2020 May 22;23(5):101109. doi:

Insert

3XMyc-EGFP-PEX26

Use

Lentiviral

Tags

Myc

Promoter

UBC

Available Since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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EGFR L858R

Plasmid

#11012

Purpose

Retroviral construct for expressing human EGFR with L858R mutation in human cells

Depositor

Matthew Meyerson

Article

Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

Insert

EGFR L858R (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

L858R (tumor-derived)

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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3X FLAG-FUS-WT

Plasmid

#44985

Depositor

Robin Reed

Article

Yamazaki et al Cell Rep. 2012 Oct 25;2(4):799-806. doi:

Insert

FUS (Full length) (FUS Human)

Tags

3X FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2013

Availability

Academic Institutions and Nonprofits only

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mTurquoise2-N1

Plasmid

#54843

Purpose

Localization: N1 Cloning Vector, Excitation: 434, Emission: 474

Depositor

Michael Davidson, Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Type

Empty backbone

Tags

mTurquoise2

Expression

Mammalian

Available Since

June 12, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFPc1 IP3R3 WT

Plasmid

#236453

Purpose

transient overexpression of IP3R3 in mammalian cells

Depositor

Michele Pagano

Article

Kuchay et al Nature. 2017 Jun 22;546(7659):554-558. d

Insert

IP3R3 (Itpr3 Rat)

Expression

Mammalian

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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