174,526 results

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pBIG1a

Plasmid

#80611

Purpose

pBIG1a - Step1 vector a (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Article

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Expression

Insect

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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p5E-elavl3

Plasmid

#75025

Purpose

Can be used to drive pan neuronal expression in zebrafish.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

ELAV like neuron-specific RNA binding protein 3 (elavl3 Zebrafish)

Use

5' entry vector for multisite gateway three …

Promoter

elavl3

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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CIB1-CreC(N1)

Plasmid

#75367

Purpose

Expresses Arabidopsis CIB1 (full length) fused to C-term fragment of Cre recombinase

Depositor

Chandra Tucker

Article

Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

Insert

CIB1 (CIB1 Mustard Weed)

Tags

Cre DNA recombinase, residues 106-343

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCDH-puro-CMV-GC3AI

Plasmid

#78910

Purpose

Indicator of caspase-3-like protease activity

Depositor

Binghui Li

Article

Zhang et al Nat Commun. 2013;4:2157. doi: 10.1038/nc

Insert

GC3AI

Use

Lentiviral

Tags

HA and Myc

Mutation

S320A, deletion of AA1-200

Promoter

CMV

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

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SS9_RNA

Plasmid

#71656

Purpose

SS9-targeting gRNA for co-transformation with pSS9

Depositor

Ryan Gill

Article

Bassalo et al ACS Synth Biol. 2016 Apr 22.

Insert

SS9 gRNA

Use

CRISPR

Expression

Bacterial

Promoter

J23119

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pGEx-4T-1-YTHDF3

Plasmid

#70088

Purpose

Encode YTHDF3 isoform a with N-terminal GST tag for E. coli expression

Depositor

Chuan He

Article

Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

Insert

YTH N(6)-methyladenosine RNA binding protein 3 (YTHDF3 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only
c-Myc-5-HT2A

Plasmid

#67944

Purpose

N-terminally c-myc tagged human 5-hydroxytryptamine receptor 2A

Depositor

Javier Gonzalez-Maeso

Article

Gonzalez-Maeso et al Nature. 2008 Mar 6;452(7183):93-7. doi:

Insert

HTR2A (HTR2A Human)

Tags

c-Myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pBAD33-Gm

Plasmid

#65098

Purpose

bacterial expression vector with Gentamycin selection, based on pBAD33

Depositor

Juan Tomas

Article

Jimenez et al J Bacteriol. 2009 Apr;191(7):2228-36. do

Type

Empty backbone

Expression

Bacterial

Promoter

pBAD

Available Since

June 5, 2015

Availability

Academic Institutions and Nonprofits only

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MSCV-Luciferase-IRES-NrasG12D

Plasmid

#60834

Purpose

Mouse NrasG12D was amplified and mutated by PCR from IMAGE clone 6475312 (Invitrogen) and cloned downstream from the IRES; subsequently Luciferase was cloned upstream of the IRES

Depositor

Scott Lowe

Article

Zuber et al Genes Dev. 2009 Apr 1;23(7):877-89. doi:

Insert

NrasG12D (Nras Mouse)

Use

Retroviral

Available Since

Nov. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pmTurquoise2-T2A-Venus(L68V)

Plasmid

#60494

Purpose

Produces equimolar levels of mTurquoise2 and mVenus(L68V). It can be used as a negative control for FRET.

Depositor

Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

mTurquoise2-T2A-mVenus(L68V)

Tags

T2A peptide

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG-mNaChBac-T2A-tdTomato

Plasmid

#60650

Purpose

Expresses mNaChBac-T2A-tdTomato

Depositor

Massimo Scanziani

Article

Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

Insert

mNaChBac-T2A-tdTomato

Expression

Mammalian

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG_mRuby2_smFP FLAG

Plasmid

#59760

Purpose

encodes multiple copies of an epitope tag FLAG ( not detected by anti GFP ab)

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Insert

mRuby2_smFP_FLAG

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 21, 2014

Availability

Academic Institutions and Nonprofits only

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p3XFLAG-CMV-14-WDR5

Plasmid

#59974

Purpose

Mammalian Expression of WDR5-FLAG

Depositor

Debu Chakravarti

Article

Kim et al Mol Cell. 2014 May 22;54(4):613-25. doi:

Insert

WDR5 (WDR5 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Oct. 8, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-CIB1-mCerulean-MP

Plasmid

#58366

Purpose

For use in light-induced protein inactivation through the interaction with CRY2

Depositor

Won Do Heo

Article

Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

Inserts

CIB1[1-147] (CIB1 Mustard Weed)
Camk2a[315-478] (Camk2a Rat)

Tags

mCerulean

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only

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p201G Cas9

Plasmid

#59178

Purpose

Cas9 driven by double 35S, GFP for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

Depositor

Wayne Parrott

Article

Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

Inserts

Cas9
sGFP

Use

CRISPR

Promoter

2x35S

Available Since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only

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mEmerald-Nesprin3-C-18

Plasmid

#54203

Purpose

Localization: Nuclear Membrane , Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

Nesprin3 (SYNE3 Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pSUMO2

Plasmid

#52259

Purpose

Bacterial expression of human 6His-tagged SUMO2, SUMO-E1 and SUMO-E2

Depositor

Primo Schaer

Article

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Inserts

SUMO-2 (SUMO2 Human)
UBE2l (UBE2I Human)
SAE-2 (UBA2 Human)
SAE-1 (SAE1 Human)

Tags

6His-tag

Expression

Bacterial

Mutation

C-terminal gly-gly

Promoter

T7 and rbs

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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pBT378_pattB-pCA-GFP-pA-attB

Plasmid

#52554

Purpose

for PhiC31 integrase-mediated cassette exchange

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attB (full length)
GFP

Expression

Mammalian

Available Since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

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pZT-AAVS1-R1

Plasmid

#52638

Purpose

TALEN-mediated gene editing at human PPP1R12C/AAVS1 locus

Depositor

Mahendra Rao, Jizhong Zou

Article

Luo et al Stem Cells Transl Med. 2014 May 15. pii:

Insert

Right AAVS1 TALEN

Use

TALEN

Expression

Mammalian

Promoter

CMV

Available Since

May 12, 2014

Availability

Academic Institutions and Nonprofits only

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pEF-Bos-FLAG RIG-I MIII

Plasmid

#52877

Purpose

Expresses RIG-I with a mutation to motif III in mammalian cells

Depositor

Curt Horvath

Article

Bamming et al J Biol Chem. 2009 Apr 10;284(15):9700-12

Insert

DDX58 (RIGI Human)

Tags

FLAG

Expression

Mammalian

Mutation

T409A, S411A

Available Since

May 9, 2014

Availability

Academic Institutions and Nonprofits only

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