159,557 results

1. pCoofy55

   Plasmid

   #122007

   Purpose

   Mammalian vector for parallel SLIC cloning containing N-terminal His10-SumoStar (Life Sensors) with additional PreScission Cleavage site and different C-tag options

   Depositor

   Sabine Suppmann

   Article

   Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

   Type

   Empty backbone

   Use

   Tags

   His10-SumoStar (Life Sensors) and Multi2: dependi…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. ND4.2-Left TALE-G1397-N-dead-mCherry

   Plasmid

   #179683

   Purpose

   Expression of base editor in mammalian cells and for cell sorting

   Depositor

   David Liu

   Article

   Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

   Insert

   COX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N (E1347A)_1xUGI_P2A_mCherry_ATP5B 3'UTR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E1347A

   Promoter

   Available since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR223_TRAF3_WT

   Plasmid

   #81755

   Purpose

   Gateway Donor vector containing TRAF3 , part of the Target Accelerator Plasmid Collection.

   Depositor

   William Hahn, David Root, Jesse Boehm

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   TRAF3 (TRAF3 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   WT

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a hMDH1v3 (+TEV)

   Plasmid

   #204229

   Purpose

   Bacterial expression of human cytosolic malate dehydrogenase isoform 1 transcript variant 3 (hMDH1v3); Use in CURES (integration of research into undergraduate teaching labs).

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Insert

   hMDH1v3 (MDH1 Human)
   

   Use

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   Inserted a G after ATG to maintain kozak site

   Promoter

   Lac

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. All-in-one lenti-BE4-NG

   Plasmid

   #200448

   Purpose

   Lentivirus vector for base editing

   Depositor

   Benjamin Cravatt

   Article

   Li et al Nat Chem Biol. 2023 Nov;19(11):1320-1330

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. MSCV-AML1/ETO-IRES-GFP

   Plasmid

   #60832

   Purpose

   AML1/ETO9a cDNA was generated by PCR from full-length human AML1/ETO-IRES-GFP and subcloned into MSCV-IRES-GFP

   Depositor

   Scott Lowe

   Article

   Zuber et al Genes Dev. 2009 Apr 1;23(7):877-89. doi:

   Insert

   AML1/ETO (RUNX1, RUNX1T1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR221_SLC25A45

   Plasmid

   #132054

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC25A45 (SLC25A45 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 4, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. IL-8 toehold switch sensor

   Plasmid

   #111908

   Purpose

   Toehold switch sensor to detect IL-8 mRNA with GFP output

   Depositor

   James Collins

   Article

   Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

   Insert

   IL8 toehold switch sensor
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Sept. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1/Ndi-1/myc-His

   Plasmid

   #127503

   Purpose

   Expresses myc-tagged yeast Ndi-1 in mammalian cells

   Depositor

   Dieter Wolf, Shashi Jain

   Article

   Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

   Insert

   Ndi-1 (NDI1 Budding Yeast)
   

   Use

   Tags

   myc-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDEST-FRT/T0-Flag-PALB2

    Plasmid

    #71114

    Purpose

    mammalian expession of FLAG-PALB2

    Depositor

    Daniel Durocher

    Article

    Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

    Insert

    PALB2 (PALB2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TO

    Available since

    Dec. 9, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. VE-cad-eGFP Donor plasmid

    Plasmid

    #92309

    Purpose

    Knock eGFP into human VE-cad locus

    Depositor

    Sean Palecek

    Article

    Bao et al Bioeng Transl Med. 2017 Jun;2(2):191-201

    Insert

    VEcad Homologous Recombination Arms (CDH5 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    about 30 a.a. truncated at end of protein

    Promoter

    Available since

    Aug. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pS-cr:GFP

    Plasmid

    #196295

    Purpose

    35S promoter-driven expression of the positive-sense TSWV S RNA segment encoding crRNA:GFP fusion in place of the NSs

    Depositor

    Zhenghe Li

    Article

    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

    Insert

    Full length TSWV S antigenome encoding crRNA:GFP fusion in place of viral NSs
    

    Use

    CRISPR

    Tags

    SpeI-DR-BsaI-DR-SpeI

    Expression

    Plant

    Mutation

    Promoter

    duplicated cauliflower mosaic virus 35S promoter

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUS212

    Plasmid

    #84580

    Purpose

    E.coli cloning and expression vector. Has cloning sites downstream of Tet promoter.

    Depositor

    Nicholas Coleman

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 21, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pLV-UbC-Tet1v4-dCas9

    Plasmid

    #232180

    Purpose

    Tet1v4-dCas9 from Nuñez et al. 2021, in a lentiviral cassette with blasticidin resistance, for expression in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Rohm et al Cell Genom. 2025 Feb 12;5(2):100770. doi

    Insert

    Tet1 catalytic domain - XTEN80 linker - dead Cas9 - 2A - BlastR (TET1 S. pyogenes, Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    hUbC

    Available since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMEH303 NLS dTomato 6x high affinity MS2 loops

    Plasmid

    #168215

    Purpose

    Mammalian expression of nuclear-localized dTomato with 6 high affinity MS2 loops

    Depositor

    Joshua Leonard

    Article

    Hung et al J Extracell Vesicles. 2016 May 13;5:3102

    Insert

    NLS dTomato
    

    Use

    Lentiviral

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEF5B-FRT-GFP-αTAT1

    Plasmid

    #27099

    Depositor

    Maxence Nachury

    Article

    Shida et al Proc Natl Acad Sci U S A. 2010 Nov 10. (

    Insert

    α-Tubulin K40 acetyltransferase1 (ATAT1 Human)
    

    Use

    Tags

    GFP, S-tag, and TEV site

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-Egr1

    Plasmid

    #11729

    Depositor

    Eileen Adamson

    Article

    Yu et al Mol Cell. 2004 Jul 2. 15(1):83-94.

    Insert

    Egr-1 (Egr1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. Mucolipin1-pEGFP C3

    Plasmid

    #62960

    Purpose

    full length human Mucolipin-1 cloned into pEGFP C3

    Depositor

    Paul Luzio

    Article

    Pryor et al Traffic. 2006 Oct;7(10):1388-98.

    Insert

    Mucolipin-1 (MCOLN1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Mucolipin-1 has 1 silent mutation compared to the…

    Promoter

    CMV

    Available since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pTRIP-CMV-GFP-FLAG-cGAS E225A-D227A

    Plasmid

    #86674

    Purpose

    Expresses catalytically-inactive GFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.

    Depositor

    Nicolas Manel

    Article

    Raab et al Science. 2016 Apr 15;352(6283):359-62. d

    Insert

    GFP-FLAG-cGAS E225A/D227A
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. p-dCas9-JMJD2A-Hygro

    Plasmid

    #104410

    Purpose

    transient expression of dCas9-JMJD2A fusion protein

    Depositor

    Stephan Beck

    Insert

    JMJD2A (KDM4A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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