159,371 results

1. pET-26b-NbVHH05-ALFA-His

   Plasmid

   #171569

   Purpose

   Bacterial expression of ALFA-His-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-ALFA-His)

   Depositor

   Hidde Ploegh, Norbert Perrimon

   Article

   Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

   Insert

   NbVHH05-ALFA-His (UBE2J1 Human)
   

   Use

   Tags

   ALFA-tag/His-tag and PelB signal peptide

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRU41

   Plasmid

   #167663

   Purpose

   ENTRY vector for gRNA1 cloning under pAtU6 promoter

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   pAtU6
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV5 HA FoxO1

   Plasmid

   #14936

   Depositor

   Joan Massague

   Article

   Seoane et al Cell. 2004 Apr 16. 117(2):211-23.

   Insert

   FoxO1 (Foxo1 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 17, 2007

   Availability

   Academic Institutions and Nonprofits only

4. (344) pcDNA3.1-HA-nGFP-(EAAAK)4-OGA(544-706)

   Plasmid

   #168097

   Purpose

   HA- tagged GFP nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGA.

   Depositor

   Christina Woo

   Article

   Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

   Insert

   HA-nGFP-(EAAAK)4-OGA(544-706)
   

   Use

   Tags

   HA-Tag

   Expression

   Mammalian

   Mutation

   Promoter

   T7/CMV

   Available since

   May 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTwist-SARS-CoV-2 Spike-deltaC18 KP.2

   Plasmid

   #223273

   Purpose

   Encodes SARS-CoV-2 spike KP.2 for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike KP.2 (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   See Depositor Comments.

   Promoter

   CAG

   Available since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSEM233 - [Pmlc-1 | tagRFP-T | cbr-tbb-2 UTR]

   Plasmid

   #159899

   Purpose

   Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

   Insert

   Pmlc-1 | tagRFP | cbr-tbb-2 UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Oct. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSyn-DIO-Gluc-RMA-IRES-EGFP

   Plasmid

   #189630

   Purpose

   Expresses Gluc-RMA and EGFP in the presence of Cre recombinase. Double-floxed Gluc-RMA and EGFP for monitoring Cre-expressing neuronal populations.

   Depositor

   Jerzy Szablowski

   Article

   Lee et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

   Inserts

   Gaussia luciferase fused to Fc
   IRES-EGFP
   

   Use

   AAV, Cre/Lox, and Luciferase

   Tags

   Gluc and IgG1 Fc

   Expression

   Mammalian

   Mutation

   Promoter

   IRES and hSyn

   Available since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. plenti-CAG-IKZF3-FLAG-IRES-GFP

   Plasmid

   #107388

   Purpose

   Lentiviral expression of IKZF3-FLAG

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   IKZF3 (IKZF3 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDEST53-LRRK2-Y1699C

   Plasmid

   #25048

   Depositor

   Mark Cookson

   Article

   Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

   Insert

   LRRK2 (LRRK2 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Tyrosine 1699 mutated to Cysteine

   Promoter

   Available since

   June 17, 2010

   Availability

   Academic Institutions and Nonprofits only

10. NET47 pEGFP-N2 (593)

    Plasmid

    #61986

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

    Insert

    NET47 (TM7SF2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pCS2_IRES2_EGFP_KCNQ2 var4

    Plasmid

    #173154

    Purpose

    For mammalian expression of KCNQ2 and creating variants

    Depositor

    Al George

    Article

    Simkin et al Elife. 2021 Feb 5;10. pii: 64434. doi: 1

    Insert

    KCNQ2 (KCNQ2 Human)
    

    Use

    Tags

    IRES2-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Simian CMV IE94

    Available since

    Sept. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. moxGFP

    Plasmid

    #68070

    Purpose

    C-terminal fusion to moxGFP

    Depositor

    Erik Snapp

    Article

    Costantini et al Nat Commun. 2015 Jul 9;6:7670. doi: 10.1

    Type

    Empty backbone

    Use

    Tags

    moxGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDisplay-SNAPtag

    Plasmid

    #205094

    Purpose

    Display SNAPtag on the surface of mammalian cells

    Depositor

    Bianxiao Cui

    Article

    Zhang et al Nat Cell Biol. 2023 Sep 28. doi: 10.1038

    Insert

    SNAPtag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PZac2.1 hSyn1-BioID2-BioID2-HA

    Plasmid

    #176855

    Purpose

    Expresses the promiscuous small biotin ligase, BioID2, in neurons

    Depositor

    Baljit Khakh

    Article

    Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

    Insert

    BioID2-BioID2-HA
    

    Use

    AAV

    Tags

    HA

    Expression

    Bacterial

    Mutation

    Promoter

    hSyn1

    Available since

    April 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGFP Kindlin2

    Plasmid

    #105305

    Purpose

    To study effect of Kindlin 2 on Cell/ECM adhesion

    Depositor

    Kenneth Yamada

    Article

    Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

    Insert

    Kindlin2 (FERMT2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. ORAI1-hBACCS2

    Plasmid

    #67626

    Purpose

    Mammalian expression of ORAI1-hBACCS2, a fusion protein of ORAI1 and human blue light-activated Ca2+ channel switch

    Depositor

    Takao Nakata

    Article

    Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

    Insert

    ORAI1-hBACCS2
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GPR101-Tango

    Plasmid

    #66301

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GPR101 (GPR101 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

18. lentiGuide-tagBFP

    Plasmid

    #217007

    Purpose

    lentiviral plasmid for gRNA cloning with selectable tagBFP expression

    Depositor

    Deepta Bhattacharya

    Article

    D'Souza et al PLoS One. 2022 Aug 24;17(8):e0261801. do

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCO605

    Plasmid

    #139738

    Purpose

    individual component of Fungal Bioluminescent Pathway, MoClo level 0 plasmid, AsNPGA

    Depositor

    Daniel Voytas

    Article

    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

    Insert

    NPGA
    

    Use

    Luciferase; Moclo level 0 component

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pWZL-hygro-FAKWT

    Plasmid

    #216540

    Purpose

    This retroviral plasmid expresses the human wild type PTK2

    Depositor

    Georgia Konstantinidou

    Article

    Pozzato et al EMBO Mol Med. 2024 Sep 13. doi: 10.1038/

    Insert

    PTK2 (PTK2 Human)
    

    Use

    Retroviral

    Tags

    No

    Expression

    Mammalian

    Mutation

    wild type sequence

    Promoter

    Available since

    Sept. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge