Inactivation of Fbxw7 impairs dsRNA sensing and confers resistance to PD-1 blockade.
Gstalder C, Liu D, Miao D, Lutterbach B, DeVine AL, Lin C, Shettigar M, Pancholi P, Buchbinder EI, Carter SL, Manos MP, Rojas-Rudilla V, Brennick R, Gjini E, Chen PH, Lako A, Rodig S, Yoon CH, Freeman GJ, Barbie DA, Hodi FS, Miles W, Van Allen EM, Haq R
Cancer Discov. 2020 May 5. pii: 2159-8290.CD-19-1416. doi: 10.1158/2159-8290.CD-19-1416. PubMed Article
Gstalder C, Liu D, Miao D, Lutterbach B, DeVine AL, Lin C, Shettigar M, Pancholi P, Buchbinder EI, Carter SL, Manos MP, Rojas-Rudilla V, Brennick R, Gjini E, Chen PH, Lako A, Rodig S, Yoon CH, Freeman GJ, Barbie DA, Hodi FS, Miles W, Van Allen EM, Haq R
Cancer Discov. 2020 May 5. pii: 2159-8290.CD-19-1416. doi: 10.1158/2159-8290.CD-19-1416. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 160090 | LentiGuide-hygro | Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker |
| 160091 | LentiGuide-zeo | Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and zeocin resistance marker |
| 160092 | pLX304_zeo | Lentiviral vector for mammalian gene expression |
| 160093 | pLX304_zeo_empty | Control lentiviral vector for mammalian gene expression |
| 160094 | pDONR221_eGFP (open) | Expresses eGFP in mammalian cells |
| 160095 | pLX304_zeo_eGFP | Expresses eGFP in mammalian cells |
| 160096 | pLX304_zeo_eGFP_V5 | Expresses eGFP in mammalian cells |
| 160097 | pDONR221_mmIrf1 (closed) | Expresses murine Irf1 in mammalian cells |
| 160098 | pLX304_zeo_mmIrf1 | Expresses murine Irf1 in mammalian cells |
| 160099 | pENTR1A_Mavs (open) | Expresses murine Mavs in mammalian cells |
| 160100 | pLX304_zeo_mmMavs_V5 | Expresses murine Mavs in mammalian cells |
| 160101 | pENTR1A_mmFbxw7a (closed) | Expresses murine Fbxw7 alpha in mammalian cells |
| 160102 | pLX304_zeo_mmFbxw7a | Expresses murine Fbxw7 alpha in mammalian cells |
| 160103 | pENTR1A_mmFbxw7_R505C (closed) | Expresses murine Fbxw7 alpha with the R505C loss-of-function mutation in mammalian cells |
| 160104 | pLX304_zeo_mmFbxw7_R505C | Expresses murine Fbxw7 alpha with the R505C loss-of-function mutation in mammalian cells |
| 160105 | pENTR1A_mmFbxw7(res) (closed) | Expresses murine Fbxw7 alpha with a T199 silent mutation in mammalian cells. The T199 silent mutation confers resistance to the 5'-TGAACATGGTACAAGGCCAG-3' sgRNA |
| 160106 | pLX304_zeo_mmFbxw7(res) | Expresses murine Fbxw7 alpha with a T199 silent mutation in mammalian cells. The T199 silent mutation confers resistance to the 5'-TGAACATGGTACAAGGCCAG-3' sgRNA |
| 160107 | pENTR223.1_mmDdx58 (closed) | Expresses murine Ddx58 in mammalian cells |
| 160108 | pLenti6.2_3xFLAG_V5_ mmDdx58_V5 | Expresses murine Ddx58 in mammalian cells |
| 160110 | pLenti6.2_3xFLAG_V5_ mmIfih1_V5 | Expresses murine Ifih1 in mammalian cells |
| 160111 | pLenti6.2_3xFLAG_V5_ eGFP_V5 | Expresses eGFP in mammalian cells |