Optimized nickase- and nuclease-based prime editing in human and mouse cells.
Adikusuma F, Lushington C, Arudkumar J, Godahewa GI, Chey YCJ, Gierus L, Piltz S, Geiger A, Jain Y, Reti D, Wilson LOW, Bauer DC, Thomas PQ
Nucleic Acids Res. 2021 Sep 17. pii: 6371974. doi: 10.1093/nar/gkab792. PubMed Article
Adikusuma F, Lushington C, Arudkumar J, Godahewa GI, Chey YCJ, Gierus L, Piltz S, Geiger A, Jain Y, Reti D, Wilson LOW, Bauer DC, Thomas PQ
Nucleic Acids Res. 2021 Sep 17. pii: 6371974. doi: 10.1093/nar/gkab792. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 171991 | PEA1-Puro | Delivers all prime editing nickase components in a single, puromycin selectable plasmid |
| 171992 | PEA1-Nuclease-Puro | Delivers all prime editing nuclease components in a single, puromycin selectable plasmid |
| 171993 | PEA1-GFP | Delivers all prime editing nickase components in a single, GFP selectable plasmid |
| 171994 | PEA1-Nuclease-GFP | Delivers all prime editing nuclease components in a single, GFP selectable plasmid |
| 171995 | PEA1-Nuclease-Puro HEK3 CTT ins | Delivers all prime editing nuclease components targeting the HEK3 gene for a CTT inserrtion, in a single, puromycin selectable plasmid |
| 171996 | PEA1-Puro-HEK3 CTT ins | Delivers all prime editing (nickase) components targeting the HEK3 site for a CTT inserrtion, in a single, puromycin selectable plasmid |
| 171997 | pCMV_T7-PE2-Nuclease | Sequence provided for PE-Nuclease mRNA transcription, suitable for microinjection |
| 176527 | PEA1 | Delivers all prime editing nickase components in a single plasmid |
| 176528 | PEA1-Nuclease | Delivers all prime editing nuclease components in a single plasmid |