159,352 results

1. Flag-TRIM28

   Plasmid

   #124960

   Purpose

   Mammalian expression of Human TRIM28

   Depositor

   Michelle Barton

   Article

   Li et al Oncogene. 2017 May 25;36(21):2991-3001.

   Insert

   hTRIM28 (TRIM28 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pL-CRISPR.EFS.GFP

   Plasmid

   #57818

   Purpose

   Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. EFS Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   SpCas9
   Sp sgRNA scaffold
   EFS (EEF1A1 Human)
   P2A-eGFP
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   EFS and hU6

   Available since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP-N1-1x

   Plasmid

   #172281

   Purpose

   Expressing EGFP mRNA fused with 1 tandem repeat of a 50-base sequence

   Depositor

   Antony K Chen

   Article

   Chen et al Sci Rep. 2017 May 8;7(1):1550. doi: 10.1

   Insert

   EGFP-N1-1x
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmScarlet3-LaminB_C1

   Plasmid

   #189776

   Purpose

   Construct for labelling nuclear envelopes in mammaiian cells using mScarlet3-LaminB fusion

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   mScarlet3-LaminB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lenti-Hb9-GFP

   Plasmid

   #37080

   Purpose

   3rd generation lentiviral transfer plasmid for Hb9 GFP

   Depositor

   Fred Gage

   Article

   Marchetto et al Cell Stem Cell. 2008 Dec 4;3(6):649-57.

   Insert

   Hb9 GFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Hb9

   Available since

   Oct. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-Syn-DIO-TRPV1-P2A-mCherry

   Plasmid

   #200831

   Purpose

   In the presence of Cre, drives neuron-specific expression of TRPV1 with fluorescence reporter mCherry

   Depositor

   Hong Chen

   Article

   Yang et al Brain Stimul. 2021 Jul-Aug;14(4):790-800

   Insert

   TRPV1-P2A-mCherry (Trpv1 Rat)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFA6a-TurboID-3MYC-natMX6 (pFB1434)

   Plasmid

   #126050

   Purpose

   For C-terminal tagging with TurboID in yeast including nourseothricin-resistant marker

   Depositor

   François Bachand

   Article

   Larochelle et al J Cell Sci. 2019 May 7. pii: jcs.232249.

   Insert

   TurboID
   

   Use

   Pcr-based c-terminal tagging

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. enOgeuIscB with ωRNA-v13

   Plasmid

   #220940

   Purpose

   CMV-driven expression of enOgeuIscB and human U6-driven expression of ωRNA-v13 scaffold with BsmBI for guide cloing

   Depositor

   Weixin Tang

   Article

   Yan et al Nat Chem Biol. 2024 Jul 8. doi: 10.1038/

   Inserts

   CMV-SV40 NLS-enOgeuIscB_nucleoplasmin NLS
   OgeuIscB-ωRNA-v13 scaffold with BsmBI for guide cloning
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPolI_SARS-CoV-2_NLuc

   Plasmid

   #183953

   Purpose

   Encodes SARS-CoV-2 minigenome with nano-luciferase reporter gene. Minigenome is under control of the Pol I promoter

   Depositor

   AJ te Velthuis

   Article

   Vial et al bioRxiv 2021.06.28.450211

   Insert

   SARS-CoV-2 minigenome with Nano luciferase (N, ORF10 SARS coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Mammalian Pol I

   Available since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. TFORF3195

    Plasmid

    #144671

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ONECUT1 (ONECUT1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40

    Plasmid

    #105540

    Purpose

    AAV expression of EGFP-Cre from hSyn promoter

    Depositor

    James M. Wilson

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    EGFP-Cre
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Feb. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBBR-Cas9ΔyqhD-HA500

    Plasmid

    #220369

    Purpose

    A single-plasmid-based, easily cured CRISPR/Cas9 system for genome engineering in Pseudomonas putida KT2440

    Depositor

    Yilan Liu

    Inserts

    SpCas9
    sgRNA for yqhD in P. putida KT2440
    

    Use

    CRISPR; Broad host range

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-EGFP-Cdc42(T17N)

    Plasmid

    #12601

    Depositor

    Klaus Hahn

    Article

    Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

    Insert

    EGFP-Cdc42 (T17N) (CDC42 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    T17N

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. IF-GFP-ATRX

    Plasmid

    #45444

    Purpose

    ATRX expression vector

    Depositor

    Michael Dyer

    Article

    Valle-Garcia et al Springerplus. 2014 May 2;3:222. doi: 10.

    Insert

    ATRX (ATRX Human, Mouse)
    

    Use

    Tags

    GFP and HA

    Expression

    Bacterial and Mammalian

    Mutation

    isoform 2, missing codon E124

    Promoter

    PCMV

    Available since

    May 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pENTR-R2L3-IRES-puro-pA

    Plasmid

    #139529

    Purpose

    Provides IRES-puro-pA cassette on Gateway entry vector

    Depositor

    Minoru Ko

    Insert

    puromycin resistant gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-hACE2-LgBiT

    Plasmid

    #215837

    Purpose

    Expresses hACE2 receptor with LgBiT tag in mammalian cells

    Depositor

    Thomas Gallagher

    Article

    Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

    Insert

    hACE2-LgBiT (ACE2 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pIZ-Flag6His-Siwi

    Plasmid

    #50560

    Purpose

    Expresses FLAG, His tagged Siwi in silkworm cell line BmN4 or insect cells

    Depositor

    Yukihide Tomari

    Article

    Kawaoka et al RNA. 2009 Jul;15(7):1258-64. doi: 10.126

    Insert

    Siwi (Aubergine )
    

    Use

    Tags

    6xHIS and FLAG

    Expression

    Insect

    Mutation

    Promoter

    OpIE2

    Available since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. prhaBAD-pA-Hia5

    Plasmid

    #222303

    Purpose

    Plasmid for rhamnose inducible expression and purification of protein A-Hia5-6XHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)

    Depositor

    Aaron Straight

    Article

    Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi

    Insert

    pA-Hia5
    

    Use

    Tags

    6His and protein A

    Expression

    Bacterial

    Mutation

    codon optimized for bacterial expression

    Promoter

    rhaBAD

    Available since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFBDM

    Plasmid

    #110738

    Purpose

    Plasmid for the insect cell expression system, two promoters, Multiplication Module M, Tn7 transposition

    Depositor

    Tim Richmond

    Article

    Fitzgerald et al Nat Methods. 2006 Dec;3(12):1021-32. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin and p10 promoters

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSEM396 - mosTI unc-119 - NNN - operon - gfp - tbb-2

    Plasmid

    #182353

    Purpose

    GFP co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::gfp::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Type

    Empty backbone

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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