163,608 results

1. pBbA5a-sufABCDSE

   Plasmid

   #195056

   Purpose

   Expresses SUF operon from E. coli from IPTG-inducible promoter

   Depositor

   Greg Bokinsky

   Article

   D'Angelo et al Elife. 2022 Mar 4;11:e70936. doi: 10.755

   Insert

   sufABCDSE
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1_FASN

   Plasmid

   #107138

   Purpose

   His tagged human FASN cloned in pcDNA3.1 vector for expression in mammalian cells

   Depositor

   Brijesh Kumar Singh

   Insert

   Fatty acid synthase (FASN Human)
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTHSSe_42

   Plasmid

   #109240

   Purpose

   pSC101 BbsI cloning vector

   Depositor

   Christopher Voigt

   Article

   Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

   Insert

   sfGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PJ23105

   Available Since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-PKA-SPARK

   Plasmid

   #106920

   Purpose

   Express PKA-SPARK, an activity reporter of protein kinase A in mammalian cells.

   Depositor

   Xiaokun Shu

   Article

   Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

   Insert

   PKA-SPARK
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFUW-tetO-Irf8

   Plasmid

   #139838

   Purpose

   doxycycline-inducible expression of mouse Irf8 in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

   Insert

   Irf8 (Irf8 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE-mCMV

   Available Since

   April 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330-PITCh-GOLGA2

   Plasmid

   #207791

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of GOLGA2 for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting N-terminus of GOLGA2 (GOLGA2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Dec. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pVITRO1-Trastuzumab-IgG4/κ

   Plasmid

   #61887

   Purpose

   Expresses HER2/neu receptor specific humanized IgG4/κ antibody isotype (Trastuzumab-IgG4/κ)

   Depositor

   Andrew Beavil

   Article

   Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

   Inserts

   HER2/neu receptor specific humanized Gamma 4 heavy chain expression cassette
   HER2/neu receptor specific humanized kappa light chain expression cassette
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mEF1 Prom and rEF1 Prom

   Available Since

   March 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMTMVi-N

   Plasmid

   #118756

   Purpose

   Intermediate plasmid to build tobacco mosaic virus recombinant clones that do not move systemically throughout the plant.

   Depositor

   Jose-Antonio Daros

   Article

   Shi et al Plant Biotechnol J. 2018 Nov 22. doi: 10

   Insert

   Tobacco mosaic virus cDNA fragment with a cloning polylinker
   

   Use

   Intermediate to build tobacco mosaic virus recomb…

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLOX-TERT-iresTK

   Plasmid

   #12245

   Purpose

   2nd generation lentiviral vector for reversible immortalization of human primary cells

   Depositor

   Didier Trono

   Article

   Salmon et al Mol Ther. 2000 Oct . 2(4):404-14.

   Insert

   TERT, HSV1-TK (TERT Human)
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   LoxP in 3' LTR.

   Promoter

   Available Since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNANOG-Luc

    Plasmid

    #25900

    Depositor

    Ren-he Xu

    Article

    Xu et al Cell Stem Cell. 2008 Aug 7. 3(2):196-206

    Insert

    NANOG promoter (NANOG Human)
    

    Use

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available Since

    Aug. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. Dendra2-CD9-10

    Plasmid

    #57705

    Purpose

    Localization: Tetraspanin/Membrane, Excitation: 490 / 553, Emission: 507 / 573

    Depositor

    Michael Davidson

    Insert

    CD9 (CD9 Human)
    

    Use

    Tags

    Dendra2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 6, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

12. CMV-Citron1

    Plasmid

    #134303

    Purpose

    Mammalian expression of direct-response citrate sensor

    Depositor

    Robert Campbell

    Article

    Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

    Insert

    Citron1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMK344 (Hygro-P2A-mAID)

    Plasmid

    #121178

    Purpose

    N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

    Insert

    Hygro-P2A-mAID
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-Aurora A

    Plasmid

    #198402

    Purpose

    Expression of EGFP-tagged Aurora A in mammalian cells

    Depositor

    Kenji Sugimoto

    Article

    Sugimoto et al Cell Struct Funct. 2002 Dec;27(6):457-67

    Insert

    Aurora A
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEF1a-IRES-Neo

    Plasmid

    #28019

    Depositor

    Thomas Zwaka

    Article

    Dejosez et al Genes Dev. 2010 Jul 15. 24(14):1479-84.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMSCV-mCherry-Syk

    Plasmid

    #50045

    Purpose

    Expression of mCherry-Syk in mammalian cells

    Depositor

    Hidde Ploegh

    Article

    Strijbis et al PLoS Pathog. 2013 Jun;9(6):e1003446. doi

    Insert

    Spleen tyrosine kinase (Syk) (Syk Mouse)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available Since

    Jan. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. mCherry-SH3(5R)

    Plasmid

    #112154

    Purpose

    Mammalian expression plasmid for SH3-5R in a vector expressing mCherry.

    Depositor

    Michael Rosen

    Article

    Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

    Insert

    SH3-5R (NCK1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    only the second SH3 domain is expressed in this c…

    Promoter

    CMV

    Available Since

    Feb. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. EGFP-BRCA1

    Plasmid

    #115491

    Purpose

    EGFP-BRCA1 expression vector. Parton clone BXY

    Depositor

    Rob Parton

    Article

    McMahon et al Elife. 2021 Jun 18;10. pii: 61407. doi:

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S1009R (See depositor comments)

    Promoter

    Available Since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET29b-H6_Streptavidin_sfGFP

    Plasmid

    #124296

    Purpose

    His6-tagged Streptavidin GFP fusion protein

    Depositor

    Mark Arbing

    Insert

    His6 tag - TEV protease site - T7 tag - Streptavidin (15-159) - superfolder GFP
    

    Use

    Tags

    His6 and T7 tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. MAP3K15-TAP

    Plasmid

    #69728

    Purpose

    Express tandem tagged MAP3K15 in cells

    Depositor

    Daniel Liebler

    Insert

    Apoptosis signal-regulating kinase 3 (MAP3K15 Human)
    

    Use

    Tags

    HA-Myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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