166,034 results

1. UAS:hGCG-ICAM(1235)- Nrxn1b;cryaa:mCherry

   Plasmid

   #218860

   Purpose

   10xUAS promoter driving expression of human glucagon-truncated ICAM-zfNrxn1b fusion protein for use in trans-Tango.

   Depositor

   Marnie Halpern

   Article

   Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

   Insert

   hGCG-ICAM(1235)-nrxn1b
   

   Expression

   Bacterial

   Available Since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLX304 CMV HA-HaloTag-FRB-sTurboID-high affinity (C)

   Plasmid

   #153005

   Purpose

   Expresses high-affinity split-TurboID C-terminal fragment in mammalian cells

   Depositor

   Alice Ting

   Article

   Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

   Insert

   sTurboID-high affinity (C) (G79)
   

   Use

   Lentiviral

   Tags

   FRB, HA, and HaloTag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHAGE-BRAF-V600E

   Plasmid

   #116204

   Purpose

   Lentiviral expression of BRAF V600E

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   BRAF (BRAF Human)
   

   Use

   Lentiviral

   Mutation

   V600E

   Available Since

   Jan. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB-GFP1-10-HygR

   Plasmid

   #223675

   Purpose

   To check the split GFP signals in mitochondria (1)

   Depositor

   Juan Melero-Martin

   Article

   Lin et al Nature. 2024 May;629(8012):660-668. doi:

   Insert

   GFP1-10
   

   Expression

   Mammalian

   Promoter

   cmv

   Available Since

   Nov. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGL3 NSE promoter

   Plasmid

   #11606

   Depositor

   Rosalyn Adam

   Article

   Kim et al Endocrinology. 2004 Feb . 145(2):613-9.

   Insert

   NSE promoter (ENO2 Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Available Since

   July 19, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-U6-sgRNA-hSyn-mCherry

   Plasmid

   #87916

   Purpose

   sgRNA expressing AAV construct with a mCherry reporter driven by hSyn promoter. (replaced the GFP in pX552 from Zhang lab with mCherry)

   Depositor

   Alex Hewitt

   Article

   Hung et al Invest Ophthalmol Vis Sci. 2016 Jun 1;57

   Insert

   mCherry
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   March 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1(+)/Luc2=tdT

   Plasmid

   #32904

   Purpose

   Expresses a fusion of firefly luciferase and tandem Tomato red fluorescent protein in mammalian cells.

   Depositor

   Christopher Contag

   Article

   Patel et al Cancer Res. 2010 Dec 15;70(24):10141-9.

   Inserts

   Firefly Luciferase
   tandem Tomato Red Fluorescent Protein
   

   Tags

   tdTomato red fluorescent protein

   Expression

   Mammalian

   Promoter

   CMV-IE and n/a

   Available Since

   Feb. 1, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. LentiCRISPR v2 mBAX

   Plasmid

   #129577

   Purpose

   CRISPR deletion of murine BAX

   Depositor

   Stephen Tait

   Article

   Lopez et al Nat Commun. 2016 Feb 2;7:10538. doi: 10.

   Insert

   gRNA vs murine BAX (Bax Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   None

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC57-TPNOX

   Plasmid

   #87853

   Purpose

   This plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.

   Depositor

   Vamsi Mootha

   Article

   Cracan et al Nat Chem Biol. 2017 Aug 7. doi: 10.1038/

   Insert

   TPNOX
   

   Tags

   C-terminal FLAG tag with a linker

   Expression

   Bacterial

   Available Since

   Aug. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. Cas9 TLS2

    Plasmid

    #196978

    Purpose

    Expresses Cas9 fused with TLS2 mobility signal that confers graft mobility. Induced by estradiol.

    Depositor

    Friedrich Kragler

    Article

    Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    Cas9 with TLS2 tag
    

    Expression

    Plant

    Promoter

    pG10-90

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. V5-GFP-APEX2-NES_pLX208

    Plasmid

    #202018

    Purpose

    expresses GFP-APEX2 in the mammalian cytosol, lentiviral vector

    Depositor

    Alice Ting

    Article

    Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

    Insert

    V5-GFP-APEX2-NES
    

    Use

    Lentiviral

    Tags

    GFP and V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pIRE4-Azi

    Plasmid

    #105829

    Purpose

    Plasmid encoding for the translational pair that enables efficent incoporation of p-Azido-phenylalanine in response to the amber codon into proteins in mammmalian cells

    Depositor

    Irene Coin

    Article

    Seidel et al Elife. 2017 Aug 3;6. doi: 10.7554/eLife.

    Inserts

    Humanized EAziRS
    4 copies of the U6-BstYam tRNA cassette
    

    Expression

    Mammalian

    Mutation

    (Y37L, D182S, F183M, L186A, D265R)TyrRS

    Promoter

    PGK and U6

    Available Since

    March 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAVS1-Tet-OsTIR1(F74G)-V5

    Plasmid

    #158664

    Purpose

    Tet-OsTIR1(F74G)-V5 donor for integration at the AAVS1 locus

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    Tet-OsTIR1(F74G) AAVS1
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    F74G

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCJ136

    Plasmid

    #162665

    Purpose

    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    codon optimized for expression in E. coli K12

    Promoter

    T7/lac

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-VSVP

    Plasmid

    #64088

    Purpose

    Expression vector for Vesicular Stomatitis Virus Indiana strain phosphoprotein gene

    Depositor

    Ian Wickersham

    Insert

    VSV phosphoprotein
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. mCherry2-N1

    Plasmid

    #54517

    Purpose

    Localization: N1 Cloning Vector, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Tags

    mCherry2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pgk-ATG-frt

    Plasmid

    #20734

    Depositor

    Rudolf Jaenisch

    Article

    Beard et al Genesis. 2006 Jan . 44(1):23-8.

    Insert

    pgk-ATG-frt-SAdpA-tetO
    

    Use

    Mouse Targeting

    Available Since

    March 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. TFORF2029

    Plasmid

    #143566

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SMARCA5 (SMARCA5 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. GFP-TREX1

    Plasmid

    #27219

    Depositor

    Judy Lieberman

    Article

    Yan et al Nat Immunol. 2010 Nov . 11(11):1005-13.

    Insert

    TREX1 (TREX1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    n/a

    Available Since

    Jan. 26, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV4-BlaM-Vpr

    Plasmid

    #21950

    Depositor

    Warner Greene

    Article

    Cavrois et al Nat Biotechnol. 2002 Nov . 20(11):1151-4

    Insert

    BlaM-Vpr (vpr Fusion of BlaM and HIV-1 Vpr)
    

    Expression

    Mammalian

    Available Since

    Oct. 1, 2009

    Availability

    Academic Institutions and Nonprofits only