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(Plasmid #183193)


Item Catalog # Description Quantity Price (USD)
Plasmid 183193 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Addgene Plasmid #52963
  • Backbone size (bp) 10183
  • Modifications to backbone
    Added another U6 promotor and scaffold with three enzyme cutting sites.
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Promoter hU6, mU6
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Cloning Information

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LentiGuide_DKO was a gift from Lang Li (Addgene plasmid # 183193 ; ; RRID:Addgene_183193)
  • For your References section:

    Generation of dual-gRNA library for combinatorial CRISPR screening of synthetic lethal gene pairs. Tang S, Wu X, Liu J, Zhang Q, Wang X, Shao S, Gokbag B, Fan K, Liu X, Li F, Cheng L, Li L. STAR Protoc. 2022 Aug 24;3(3):101556. doi: 10.1016/j.xpro.2022.101556. eCollection 2022 Sep 16. 10.1016/j.xpro.2022.101556 PubMed 36060092