PurposeExpresses enCas9 fused to PolI5M in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||113078||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Total vector size (bp) 12546
Vector typeBacterial Expression, CRISPR
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberLow Copy
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Plasmid contains nonsense mutation in the chloramphenicol acetyltransferase.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEvolvR-enCas9-PolI5M was a gift from John Dueber & David Schaffer (Addgene plasmid # 113078 ; http://n2t.net/addgene:113078 ; RRID:Addgene_113078)
For your References section:CRISPR-guided DNA polymerases enable diversification of all nucleotides in a tunable window. Halperin SO, Tou CJ, Wong EB, Modavi C, Schaffer DV, Dueber JE. Nature. 2018 Aug 1. pii: 10.1038/s41586-018-0384-8. doi: 10.1038/s41586-018-0384-8. 10.1038/s41586-018-0384-8 PubMed 30069054