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pMDC32-HPB
(Plasmid #32078)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 32078 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMDC32
  • Backbone size (bp) 12096
  • Modifications to backbone
    HPB tag was added to allow its fusion to gene of interest for expression in plant.
  • Vector type
    Gateway destination binary vector
  • Promoter 35S promoter
  • Selectable markers
    Hygromycin
  • Tag / Fusion Protein
    • HA-Prescission-Biotin (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DB3.1
  • Copy number
    High Copy

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMDC32-HPB was a gift from Fumi Katagiri (Addgene plasmid # 32078 ; http://n2t.net/addgene:32078 ; RRID:Addgene_32078)
  • For your References section:

    Purification of low-abundance Arabidopsis plasma-membrane protein complexes and identification of candidate components. Qi Y, Katagiri F. Plant J. 2009 Mar;57(5):932-44. Epub 2008 Dec 4. 10.1111/j.1365-313X.2008.03736.x PubMed 19000159