Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

176,159 results

Showing: 6321 - 6340 of 176159 results

  1. RED

    Plasmid
    #139835
    Purpose
    Expresses RNase H1-EGFP- dCas9 fusion protein in human cells
    Depositor
    Karim Mekhail
    Publication
    Abraham et al Nature. 2020 Sep;585(7824):298-302. doi:
    Insert
    RNase H1-EGFP-dCas9 (RNASEH1 RNase H1 (H. sapiens), dCas9 (S. pyogenes))
    Expression
    Mammalian
    Mutation
    dCas9 protein contains two mutations: D10A and H8…
    Promoter
    tet-inducible CMV
    Available since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    139835-plasmid-map-sequence-id-310615

    Enlarge

  9. 24xMoonTag-kif18b-STOP-24xSunTag-linker-24xPP7 (3’UTR translation reporter)

    Plasmid
    #128605
    Purpose
    Expresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene, STOP codon, 24xgcn4 peptide array (SunTag peptide array), linker and 24 repeats of PP7 hairpins.
    Depositor
    Marvin Tanenbaum
    Publication
    Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304
    Inserts
    24xMoonTag
    kinesin family member 18b (KIF18B Human)
    24xSunTag
    Expression
    Mammalian
    Promoter
    Pcmv
    Available since
    Jan. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    128605-plasmid-map-sequence-id-318004

    Enlarge

  15. pHAGE-GFP

    Plasmid
    #106281
    Purpose
    A version of the pHAGE vector with ZsGreen dsRed expressed separated by an IRES. Note that there is little-to-no expression of dsRed.
    Depositor
    Jay Shendure
    Publication
    Hill et al Nat Methods. 2018 Feb 19. pii: nmeth.460
    Insert
    CMV-GFP-IRES-DSRED(NOT-EXPRESSED)-WPRE-MCS
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    106281-plasmid-map-sequence-id-212556

    Enlarge

  19. pSpCas9(BB)-2A-miRFP670

    Plasmid
    #91854
    Purpose
    Cas9 from S. pyogenes with 2A-miRFP670, and cloning backbone for sgRNA. Modified Zhang Plasmid #62988
    Depositor
    Ralf Kuehn
    Inserts
    hSpCas9-2A-miRFP670
    BB-guide RNA
    Use
    CRISPR
    Tags
    2A-miRFP670 and 3XFLAG
    Expression
    Mammalian
    Promoter
    Cbh and U6
    Available since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    91854-plasmid-map-sequence-id-176529

    Enlarge

  20. TLCV2-RB1

    Plasmid
    #87836
    Purpose
    LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive expression of an sgRNA targeting human RB1.
    Depositor
    Adam Karpf
    Publication
    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:
    Insert
    sgRB1 (RB1 Human)
    Use
    CRISPR and Lentiviral; Doxycycline inducible; egf…
    Expression
    Mammalian
    Promoter
    Tight TRE promoter and U6
    Available since
    April 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    87836-plasmid-map-sequence-id-176310

    Enlarge

Showing: 6321 - 6340 of 176159 results