Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

163,584 results

Showing: 6161 - 6180 of 163584 results

  1. pNEU-hMbPylRS-4xU6M15

    Plasmid
    #105830
    Purpose
    Plasmid encoding for the Pyrrolysine translational pair that enables efficent incoporation of Pyl-like click amino acids in response to the amber codon into proteins in mammalian cells
    Depositor
    Irene Coin
    Article
    Serfling et al Nucleic Acids Res. 2017 Nov 21. pii: 464
    Inserts
    Humanized MbPylRS
    tRNAM15
    Use
    Tags
    Expression
    Mammalian
    Mutation
    (Y271A, Y349F)MbPylRS
    Promoter
    CMV and U6
    Available Since
    March 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    105830-plasmid-map-sequence-id-466939

    Enlarge

  2. pcDNA5/FRT/TO-GAlphai2-RLuc8

    Plasmid
    #140974
    Purpose
    Encodes a G alpha subunit (GNAI2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlphai2-Rluc8 (GNAI2 Human)
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140974-plasmid-map-sequence-id-300657

    Enlarge

  6. pMDlg/pRRE-int-MS2M

    Plasmid
    #166032
    Purpose
    Expression of bacteriophage-derived MS2 coat protein fused HIV-1 Lentiviral Gag-Pol at C terminal with a HIV-1 protease cleavage signal between MS2 and Gag-Pol.
    Depositor
    Yujia Cai
    Article
    Ling et al Nat Biomed Eng. 2021 Jan 4. pii: 10.1038
    Insert
    Gag-Pol-MS2
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    D64V mutation within the integrase within Pol
    Promoter
    Available Since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    166032-plasmid-map-sequence-id-338747

    Enlarge

  10. pAAV-hSyn-GRAB_g5-HT3.0

    Plasmid
    #208717
    Purpose
    Expresses the green 5-HT sensor GRAB_g5-HT3.0 in neurons
    Depositor
    Yulong Li
    Article
    Deng et al bioRxiv 2023.05.27.542566
    Has Service
    AAV1, AAV2, and AAV8
    Insert
    Green fluorescent 5-HT sensor GRAB_g5-HT3.0
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    hSyn
    Available Since
    Oct. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    208717-plasmid-map-sequence-id-412322

    Enlarge

  14. pLenti.CAG.H2B-cerFP.W.SINloxP

    Plasmid
    #51006
    Purpose
    Lentivector that expresses H2B-cerFP from internal CAG enhancer-promoter
    Depositor
    Rusty Lansford
    Insert
    H2B-cerFP
    Use
    Lentiviral
    Tags
    histone 2B
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available Since
    June 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    51006-plasmid-map-sequence-id-77912

    Enlarge

  16. DRD1-Tango

    Plasmid
    #66268
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    DRD1 (DRD1 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66268-plasmid-map-sequence-id-245407

    Enlarge

  18. pCMV-T7-ABE8e-nSpRY-P2A-EGFP (KAC1069)

    Plasmid
    #185912
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    human codon optimized ABE8e-nSpRY-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219…
    Promoter
    CMV and T7
    Available Since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185912-plasmid-map-sequence-id-367362

    Enlarge

Showing: 6161 - 6180 of 163584 results