161,641 results

1. pAcBac1-Pyl-TAG

   Plasmid

   #228784

   Purpose

   Pyl aaRS/tRNA system for mammalian TAG suppression

   Depositor

   Abhishek Chatterjee

   Article

   Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

   Inserts

   PylRS
   PytR
   

   Use

   Baculoviral

   Tags

   Expression

   Mammalian

   Mutation

   TAG suppressor

   Promoter

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. AiP13041 - pAAV-AiE0774m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3041)

   Plasmid

   #224026

   Purpose

   AiE0774m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L6_IT_Car3 neurons (note CLA neurons)

   Depositor

   Jonathan Ting, The Allen Institute for Brain Science

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   SYFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   NA

   Promoter

   minBG

   Available since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pD649-HAsp-SEMA5A-Fc(DAPA)-AviTag-6xHis

   Plasmid

   #157008

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   SEMA5A (SEMA5A Human)
   

   Use

   Tags

   Fc(DAPA)-AviTag-6xHis

   Expression

   Mammalian

   Mutation

   Fc region of human IgG contains D265A and P329A m…

   Promoter

   CMV/SP6

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSilencer2.1-U6-3JB1F+6

   Plasmid

   #190835

   Purpose

   For mammalian expression of 3JB1F+6, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.

   Depositor

   Gerald Hart

   Article

   Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

   Insert

   3JB1F_T1+6bp (A Dual-Specificity aptamer targeting OGT and GFP)
   

   Use

   Affinity Reagent/ Antibody and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 9, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. qTAG-C-mStayGold-EFS-Puro

   Plasmid

   #227256

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-EFS-Puro cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBA559

   Plasmid

   #186235

   Purpose

   pTet-LbuCas13a crRNA entry vector

   Depositor

   Jennifer Doudna

   Article

   Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMXs_FLAG-NMNAT1

   Plasmid

   #133259

   Purpose

   Express FLAG-tagged NMNAT1 in mammalian cells

   Depositor

   David Sabatini

   Article

   Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

   Insert

   NMNAT1 (NMNAT1 Human)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG mitoYFP

   Plasmid

   #168508

   Purpose

   Expression of mito-EYFP (mitochondrial matrix targeted EYFP) for visualization of mitochondrial dynamics

   Depositor

   Franck Polleux

   Article

   Lewis TL et al Curr Biol. 2016 Oct 10;26(19):2602-2608.

   Insert

   mitoYFP
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-22b DT 51E/148K

   Plasmid

   #11081

   Depositor

   John Collier

   Insert

   DT
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   51E/148K, catalytically inactive

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. LZRS-DNMT1

    Plasmid

    #24952

    Depositor

    Paul Khavari

    Article

    Sen et al Nature. 2010 Jan 28. 463(7280):563-7.

    Insert

    DNMT1 (DNMT1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CMV-FLEX-SaCas9-U6-sgRosa26

    Plasmid

    #159914

    Purpose

    Mutagenesis of Rosa26 with SauCas9

    Depositor

    Larry Zweifel

    Insert

    Rosa26 gRNA (Gt(ROSA)26Sor Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV-IRF9

    Plasmid

    #71452

    Purpose

    Lentiviral expression of IRF9

    Depositor

    George Stark

    Article

    Cheon et al EMBO J. 2013 Oct 16;32(20):2751-63. doi:

    Insert

    IRF9 (IRF9 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. U6.3>gRNA.f+e

    Plasmid

    #99139

    Purpose

    Empty gRNA scaffold with "Flip" and "Extension" modifications with optimized chicken specific U6 promoter

    Depositor

    Marianne Bronner

    Article

    Gandhi et al Dev Biol. 2017 Dec 1;432(1):86-97. doi:

    Insert

    gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    chicken U6.3

    Available since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR-CD86

    Plasmid

    #223604

    Purpose

    Lentiviral expression of human CD86 in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

    Insert

    CD86 (CD86 Human)
    

    Use

    Lentiviral; mammalian expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. MSCV IRES Luciferase

    Plasmid

    #18760

    Depositor

    Scott Lowe

    Type

    Empty backbone

    Use

    Luciferase and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti PGK GFP Puro (w509-5)

    Plasmid

    #19070

    Purpose

    3rd gen lentiviral eGFP expression vector, PGK promoter, Puro 

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    enhanced green fluorescent protein
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-30a-L6KD-PT linker-PEP-Mtu ΔI-CM-xylanase

    Plasmid

    #231128

    Purpose

    Expresses xylanase via a cSAT2.0 scheme

    Depositor

    Xiaofeng Yang

    Article

    Huang et al Trends Biotechnol. 2024 Nov 12:S0167-779

    Insert

    xylanase
    

    Use

    Tags

    cSAT2.0

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. HBV 1.3-mer X-null replicon

    Plasmid

    #65461

    Purpose

    containing 1.3 units of the X-null HBV genome (subtype ayw)

    Depositor

    Wang-Shick Ryu

    Article

    Hussain et al J Gen Virol. 2009 Sep;90(Pt 9):2272-81.

    Insert

    1.3 units of the X-null HBV genome
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. Clover2-H2B-6

    Plasmid

    #56536

    Purpose

    Localization: Nucleus/Histones, Excitation: 505, Emission: 515

    Depositor

    Michael Davidson

    Insert

    H2B (H2BC11 Human)
    

    Use

    Tags

    Clover2

    Expression

    Mammalian

    Mutation

    D26G and V119I in H2B

    Promoter

    CMV

    Available since

    Dec. 5, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pPB_TRE3G_scFV-sfGFP-Ring1bCD

    Plasmid

    #235574

    Purpose

    Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Ring1b fused with scFV to programme H2AK119ub.

    Depositor

    Jamie Hackett

    Article

    Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

    Insert

    Ring1b (Rnf2 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge