159,333 results

1. pHR-tdMCP-YFP

   Plasmid

   #99151

   Purpose

   Expresses dimeric MS2-coat binding protein tagged with YFP, 2nd generation lentiviral vector

   Depositor

   Ron Vale

   Article

   Jain et al Nature. 2017 Jun 8;546(7657):243-247. do

   Insert

   Dimeric MS2-coat binding protein
   

   Use

   Lentiviral

   Tags

   eYFP

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Aug. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGGS-Raichu-RhoA-CR

   Plasmid

   #40258

   Depositor

   Michael Lin

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Insert

   Raichu-RhoA-CR (RHOA Synthetic, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDY0181 Cargo EGFP with AttP Bxb1 site (Parent minicircle)

   Plasmid

   #179115

   Purpose

   Cargo EGFP with AttP Bxb1 site (Parent minicircle)

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

   Insert

   Cargo EGFP with AttP Bxb1 site
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmScarlet3-LaminB_C1

   Plasmid

   #189776

   Purpose

   Construct for labelling nuclear envelopes in mammaiian cells using mScarlet3-LaminB fusion

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   mScarlet3-LaminB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-RAM-d2TTA::TRE-FLEX-tdTomato-WPREpA

   Plasmid

   #84468

   Purpose

   pAAV-CRAM-tdTomato

   Depositor

   Yingxi Lin

   Article

   Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

   Insert

   tdTomato
   

   Use

   AAV

   Tags

   Expression

   Mutation

   T230I (please see depositor comments below)

   Promoter

   TRE

   Available since

   Dec. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAL119-TK

   Plasmid

   #21911

   Depositor

   Maria Castro

   Article

   Dewey et al Nat Med. 1999 Nov . 5(11):1256-63.

   Insert

   HSV Type 1 thymidine kinase
   

   Use

   Adenoviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 21, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pML433

   Plasmid

   #177190

   Purpose

   Expresses recombinant HyPro enzyme in E. coli

   Depositor

   Eugene Makeyev

   Article

   Yap et al Mol Cell. 2021 Nov 2. pii: S1097-2765(21

   Insert

   HyPro enzyme
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. M43: pHR-SFFVp-iLID::EGFP::FTH1

   Plasmid

   #122149

   Purpose

   Self-assebled 24-mer tagged with iLID, and EGFP. Upon blue-light activation can bind up to 24 SspB-modified proteins.

   Depositor

   Clifford Brangwynne

   Article

   Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

   Insert

   FTH1 (FTH1 Human)
   

   Use

   Lentiviral

   Tags

   NLS-iLID-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   March 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJKR-H-tetR

   Plasmid

   #62561

   Purpose

   Tetracycline biosensor. High copy plasmid that expresses GFP proportional to tetracycline concentration.

   Depositor

   George Church

   Article

   Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

   Insert

   GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. SpyCatcher003 S49C

    Plasmid

    #133448

    Purpose

    Expresses SpyCatcher003 S49C in bacterial cytoplasm, with a unique cysteine for chemical coupling

    Depositor

    Mark Howarth

    Article

    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

    Insert

    SpyCatcher003 S49C
    

    Use

    Tags

    GSSGS, His6, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Contains S49C mutation

    Promoter

    T7

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-Myc-IQGAP1

    Plasmid

    #30118

    Depositor

    David Sacks

    Article

    Ho et al J Biol Chem. 1999 Jan 1. 274(1):464-70.

    Insert

    IQGAP1 (IQGAP1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. siRNA-resistant SigmaR1-mCherry

    Plasmid

    #226567

    Purpose

    Encodes siRNA resistant SigmaR1 protein labeled with mCherry

    Depositor

    Gia Voeltz

    Article

    Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

    Insert

    SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLAMP1-emiRFP670

    Plasmid

    #136570

    Purpose

    LAMP1 (Lysosome Membrane) labeling with near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)

    Depositor

    Vladislav Verkhusha

    Article

    Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

    Insert

    LAMP1-emiRFP670
    

    Use

    Tags

    emiRFP670

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. Kras-Src FRET biosensor

    Plasmid

    #78302

    Purpose

    FRET biosensor. To monitor Src kinase activity in mammalian cells by FRET

    Depositor

    Yingxiao Wang

    Article

    Ouyang et al Proc Natl Acad Sci U S A. 2008 Sep 23;10

    Insert

    Src biosensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEGFP-puro

    Plasmid

    #45561

    Depositor

    Michael McVoy

    Article

    Abbate et al Biotechniques. 2001 Aug;31(2):336-40.

    Insert

    Puromycin N-acetyl transferase
    

    Use

    Bifunctional selection protein

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Phcmv (Strong human cytomegalovirus immediate ear…

    Available since

    June 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pWZL Neo Myr Flag PIK4CA

    Plasmid

    #20577

    Depositor

    Jean Zhao, William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    PIK4CA (PI4KA Human)
    

    Use

    Retroviral

    Tags

    Flag and Myr

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

17. Flag-TRIM28

    Plasmid

    #124960

    Purpose

    Mammalian expression of Human TRIM28

    Depositor

    Michelle Barton

    Article

    Li et al Oncogene. 2017 May 25;36(21):2991-3001.

    Insert

    hTRIM28 (TRIM28 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-mNox4

    Plasmid

    #69353

    Purpose

    expresses mouse Nox4 in mammalian cells

    Depositor

    Karl-Heinz Krause

    Article

    Serrander et al Biochem J. 2007 Aug 15;406(1):105-14.

    Insert

    mNox4 (Nox4 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 15, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pTRE-tdTomato

    Plasmid

    #50798

    Purpose

    Expression of tdTomato under the control of the TRE-promoter

    Depositor

    Connie Cepko

    Article

    Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

    Insert

    TREtight driven tdTomato fluorescent protein
    

    Use

    Mutli-species

    Tags

    Expression

    Mutation

    Promoter

    TREtight

    Available since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-PARP1-3xFlag-WT

    Plasmid

    #111575

    Purpose

    Expresses full length PARP1 in mammalian cells

    Depositor

    Thomas Muir

    Article

    Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

    Insert

    PARP1 (PARP1 Human)
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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