165,688 results

1. pJMK001

   Plasmid

   #33780

   Depositor

   Adam Cohen

   Article

   Kralj et al Science. 2011 Jul 15;333(6040):345-8.

   Insert

   Proteorhodopsin Optical Proton Sensor
   

   Expression

   Bacterial

   Mutation

   D97N

   Promoter

   AraBAD

   Available Since

   Dec. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. Z-lock cofilin

   Plasmid

   #141137

   Purpose

   controlling the actin-severing ability of cofilin with light

   Depositor

   Klaus Hahn

   Article

   Stone et al Nat Chem Biol. 2019 Dec;15(12):1183-1190

   Insert

   Zdk2, Cofilin, LOV2
   

   Expression

   Mammalian

   Mutation

   mCherry, Zdk2(132F), Cofilin S3A, LOV WT

   Available Since

   Dec. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. 6xHis-SNAP-PI(4,5)P2 Probe

   Plasmid

   #211513

   Purpose

   For expression of recombinant biosensor for detection of Phosphatidylinositol-4,5-Bisphosphate (PI(4,5)P2) from bacteria

   Depositor

   David Murray, Hannes Maib

   Article

   Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

   Insert

   PLCdelta1-PH
   

   Tags

   6xhis-SNAP

   Expression

   Bacterial

   Available Since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. HOP-flash

   Plasmid

   #83467

   Purpose

   TEAD-YAP/TAZ tx activity reporter. 8xwt TEAD binding sites with minimal promoter plus luciferase reporter gene

   Depositor

   Barry Gumbiner

   Article

   Kim et al J Cell Biol. 2015 Aug 3;210(3):503-15. d

   Insert

   Multimerized TEAD binding sites in the promoter of CTGF with minimal promoter (CCN2 Human)
   

   Use

   Luciferase

   Available Since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. RUSH reporter-HMGB1-SBP-GFP

   Plasmid

   #172357

   Purpose

   Monitoring of HMGB1 subcellular localization by fluorescence videomicroscopy

   Depositor

   Guido Kroemer

   Article

   Liu et al Sci Rep. 2017 Nov 2;7(1):14915. doi: 10.

   Insert

   high mobility group box 1 (HMGB1 Human)
   

   Use

   Lentiviral

   Tags

   SBP-GFP

   Promoter

   CMV

   Available Since

   June 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRRL_SRSF2_P95H_mCherry

   Plasmid

   #84023

   Purpose

   expression of SRSF2 mutant in mammalian cells

   Depositor

   Robert Bradley

   Article

   Kim et al Cancer Cell. 2015 May 11;27(5):617-30. d

   Insert

   SRSF2 (SRSF2 Human)
   

   Use

   Lentiviral

   Tags

   2TA-mCherry and Flag

   Expression

   Mammalian

   Mutation

   P95H

   Promoter

   PGK

   Available Since

   Oct. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. Cas9-T6N (pZZ12, TAT-4MycNLS-Cas9-2SV40NLS-sfGFP)

   Plasmid

   #199604

   Purpose

   Expression of cell permeable spCas9 in E. Coli.

   Depositor

   Junwei Shi

   Article

   Zhang et al Nat Biotechnol. 2023 Apr 24. doi: 10.103

   Insert

   Cas9-T6N
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. METTL3 shRNA 2 tet pLKO puro

   Plasmid

   #162984

   Purpose

   Tet-inducible shRNA targeting human METTL3 #2

   Depositor

   Mark Rubin

   Article

   Cotter et al Mol Cancer Res. 2021 Jun 4. pii: 1541-77

   Insert

   methyltransferase like 3 (METTL3 Human)
   

   Use

   Lentiviral

   Available Since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. mEmerald-Vimentin-C-18

   Plasmid

   #54300

   Purpose

   Localization: Intermediate Filaments, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Insert

   Vimentin (VIM Human)
   

   Tags

   mEmerald

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 16, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

10. tau exon 9–12 splicing minigene

    Plasmid

    #121165

    Purpose

    Generates circular RNAs from a Tau minigene

    Depositor

    Stefan Stamm

    Article

    Welden et al Biochim Biophys Acta Mol Basis Dis. 2018

    Insert

    Tau exons 9–12 (MAPT Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAX APP C99

    Plasmid

    #30146

    Depositor

    Dennis Selkoe, Tracy Young-Pearse

    Article

    Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

    Insert

    APP C99 (APP Human)
    

    Expression

    Mammalian

    Mutation

    C-terminal from BACE site of APP

    Available Since

    July 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. GFP-ERK1

    Plasmid

    #14747

    Depositor

    Rony Seger

    Article

    Yung et al J Biol Chem. 2001 Sep 21. 276(38):35280-

    Insert

    ERK1 (MAPK3 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    June 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBABE-neo largeTcDNA

    Plasmid

    #1780

    Purpose

    Retroviral expression of Large T Antigen. Used to create immortalized cell lines.

    Depositor

    Bob Weinberg

    Article

    Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

    Insert

    large T antigen (SV40gp6 simian virus)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    June 3, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFH2.9_MMLV_Gag-P2A-eGFP

    Plasmid

    #205524

    Purpose

    Express MMLV Gag-P2A-eGFP

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    Gag
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAVS1-Bxb1-LP-v2-TC

    Plasmid

    #194326

    Purpose

    AAVS1 targeting construct with the Bxb1 landing pad version 2 cassette.

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Stem Cell Res. 2023 Feb;66:102991. doi:

    Insert

    loxP-EBFP2-attP-BleoR-lox251
    

    Use

    Cre/Lox and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. NFAT luciferase reporter

    Plasmid

    #10959

    Depositor

    Toren Finkel

    Article

    Ichida et al J Biol Chem. 2001 Feb 2. 276(5):3524-30.

    Insert

    IL-2 promoter (IL2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Available Since

    Jan. 6, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV MP-135 mRFP

    Plasmid

    #206927

    Purpose

    The micro promoter MP-135 is an AAV specific strong promoter for gene expression in mammalian cells. The small size of the promoter (135 bp) allows delivery of large genes in AAV.

    Depositor

    Markus Grompe

    Article

    Chai et al Mol Ther Methods Clin Dev. 2023 May 17;2

    Insert

    mRFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    MP-135

    Available Since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. plenti.CamKII.(synapto).iATPSnFR2.A95A.A119L.HaloTag

    Plasmid

    #209729

    Purpose

    Expresses presynaptic low affinity ratiometric ATP sensor

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    (synapto).iATPSnFR2.A95A.A119L.HaloTag
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CamKII

    Available Since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. HA-SOCS1

    Plasmid

    #174571

    Purpose

    Human HA-tagged SOCS1 expression (N-term. tag) (CMV prom.)

    Depositor

    Mathew Garnett

    Article

    Coelho et al Cancer Cell. 2023 Feb 13;41(2):288-303.e

    Insert

    HA-SOCS1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. PB-PEmax

    Plasmid

    #187893

    Purpose

    All-in-one prime editor piggyBac transposon with PEmax

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

    Insert

    SpCas9_H840A_KK-MMLV-RT_co-P2A-PAC_dTK
    

    Use

    CRISPR and Synthetic Biology; Piggybac transposon

    Tags

    BPSV40 NLS and c-Myc NLS

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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