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pET/ELP-I-CAT
(Plasmid #71461)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 71461 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET21(+)
  • Backbone manufacturer
    Novagen
  • Modifications to backbone
    Added ELP and intein tags
  • Vector type
    Bacterial Expression
  • Tags / Fusion Proteins
    • ELP ((VPGXG)110) (N terminal on backbone)
    • intein (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ sequencing primer T7 to sequence ELP
  • 3′ sequencing primer F1ori-R
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

This plasmid contains the CAT gene, which can be replaced with your gene of interest using the unique BsrG I site (TGTACA) at the end of the intein and one of several sites downstream from the product protein (typically Hind III). More info on cloning can be found in supplemental document ELP cloning manual.

Please NOTE- the Addgene diagnostic digest result confirms the presence of ELP-intein.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET/ELP-I-CAT was a gift from David Wood (Addgene plasmid # 71461 ; http://n2t.net/addgene:71461 ; RRID:Addgene_71461)
  • For your References section:

    Simple bioseparations using self-cleaving elastin-like polypeptide tags. Banki MR, Feng L, Wood DW. Nat Methods. 2005 Sep;2(9):659-61. 10.1038/nmeth787 PubMed 16074986