|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||71461||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Modifications to backboneAdded ELP and intein tags
Vector typeBacterial Expression
/ Fusion Proteins
- ELP ((VPGXG)110) (N terminal on backbone)
- intein (N terminal on backbone)
Growth in Bacteria
Copy numberLow Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7 to sequence ELP
- 3′ sequencing primer F1ori-R (Common Sequencing Primers)
This plasmid contains the CAT gene, which can be replaced with your gene of interest using the unique BsrG I site (TGTACA) at the end of the intein and one of several sites downstream from the product protein (typically Hind III). More info on cloning can be found in supplemental document ELP cloning manual.
Please NOTE- the Addgene diagnostic digest result confirms the presence of ELP-intein.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pET/ELP-I-CAT was a gift from David Wood (Addgene plasmid # 71461 ; http://n2t.net/addgene:71461 ; RRID:Addgene_71461)
For your References section:Simple bioseparations using self-cleaving elastin-like polypeptide tags. Banki MR, Feng L, Wood DW. Nat Methods. 2005 Sep;2(9):659-61. 10.1038/nmeth787 PubMed 16074986