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Addgene

pET/ELP-I-CAT Notes

Addgene diagnostic digest

This gel depicts the NEB 1kb Extend DNA ladder and a digestion of plasmid #71461 with the enzymes Eco RI-HF and Nde I.

We expected the following band sizes:

EcoRI-HF / Nde I double digest: expect 2 bands: 6559bp and 1733bp

We see the expected pattern. This result verifies the presence of the ELP-intein.