This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pDiGc
(Plasmid #59322)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 59322 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Growth instructions
    Bacteria are better induced for dsRed after O/N in minimum medium (MgMES medium is ideal: 170mM 2-[N-morpholino]ethane-sulphonic acid at pH 5.0, 5 mM KCl, 7.5 mM (NH4)2SO4, 0.5 mM K2SO4, 1 mM KH2PO4, 10 mM MgCl2, 38 mM glycerol and 0.1% casamino acids (adapted from Beuzone et al., 1999)) in presence of arabinose (0.2% w/v)
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    EGFP
  • Alt name
    gpfmut3a
  • Promoter rpsM

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    dsRed
  • Alt name
    DsRed.T3_S4T
  • Promoter Pbad

Cloning Information for Gene/Insert 2

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDiGc was a gift from Sophie Helaine & David Holden (Addgene plasmid # 59322 ; http://n2t.net/addgene:59322 ; RRID:Addgene_59322)
  • For your References section:

    Dynamics of intracellular bacterial replication at the single cell level. Helaine S, Thompson JA, Watson KG, Liu M, Boyle C, Holden DW. Proc Natl Acad Sci U S A. 2010 Feb 23;107(8):3746-51. doi: 10.1073/pnas.1000041107. Epub 2010 Feb 2. 10.1073/pnas.1000041107 PubMed 20133586