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pLminP_Luc2P_RE56

Plasmid

#247034

Purpose

Glucocorticoid Receptor pathway reporter; lentiviral expression of NR3C1 (GR) DNA response element cloned 5' to firefly luciferase. Coexpresses EmGFP to monitor lentiviral transduction.

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

NR3C1 (GR)

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pLXSN16E6E7

Plasmid

#52394

Purpose

The plasmid, pLXSN16E6E7, is used to produce amphotropic recombinant retrovirus providing efficient gene transfers and expression of the HPV16 E6 and E7 genes.

Depositor

Denise Galloway

Article

Halbert et al J Virol. 1991 Jan;65(1):473-8.

Insert

HPV16 E6E7

Use

Retroviral

Available Since

May 27, 2014

Availability

Academic Institutions and Nonprofits only

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pAGM8031

Plasmid

#48037

Depositor

Sylvestre Marillonnet

Article

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available Since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-IRES-Gβγ-BERKY3

Plasmid

#158426

Purpose

BRET biosensor for detection of free Gβγ in lentiviral vector pLVX-IRES-Hyg

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

Lyn11-Nluc-ER/K linker-3xYFP-GRK3ct

Use

Lentiviral

Promoter

CMV

Available Since

Sept. 17, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5/FRT/TO-GAlphaQ-RLuc8

Plasmid

#140982

Purpose

Encodes a G alpha subunit (GNAQ) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

Depositor

Bryan Roth

Article

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Insert

GAlphaQ-RLuc8 (GNAQ Human)

Use

Luciferase

Expression

Mammalian

Mutation

RLuc8 and flanking SGGGGS linkers have been inser…

Promoter

CMV

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pET-His6MBP-A3A-Gly6His6

Plasmid

#187822

Purpose

Expression human APOBEC3A in E.coli.

Depositor

Hao Wu

Article

Fabyanic et al Nat Biotechnol. 2023 Aug 28. doi: 10.103

Insert

APOBEC3A (APOBEC3A Human)

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Mutation

none

Promoter

T7 promoter (on the backbone)

Available Since

July 21, 2023

Availability

Academic Institutions and Nonprofits only

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WNK2 WT

Plasmid

#24569

Depositor

Joseph Costello

Article

Hong et al Proc Natl Acad Sci U S A. 2007 Jun 26. 1

Insert

WNK2 (WNK2 Human)

Tags

His6 and V5

Expression

Mammalian

Available Since

May 20, 2010

Availability

Academic Institutions and Nonprofits only

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LBR-mCherry

Plasmid

#172446

Purpose

Expression of nuclear envelope protein LBR with a C-terminal mCherry tag

Depositor

Stephen Royle

Article

Ferrandiz et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

lamin B receptor (LBR Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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1XDR4

Plasmid

#32614

Depositor

Connie Cepko

Article

Billings et al PLoS One. 2010 . 5(10):e13739.

Insert

1XDR4

Expression

Mammalian

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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pLGR002 LGR Cas9 guide vector

Plasmid

#188320

Purpose

Universal backbone for CRISPR/Cas9 sgRNA expression

Depositor

Laralynne Przybyla

Article

Heo et al Genome Biol. 2024 Jan 19;25(1):25. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

PuroR-T2A-mTagBFP2

Expression

Mammalian

Promoter

pU6 5'CMV and EF1-alpha

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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22l-COX8-3xFlag-TALE(RNR2-Right)-TadA(8e+V106W)

Plasmid

#209296

Purpose

mitoBE expression vector, target mtRNR2 gene.

Depositor

Wensheng Wei

Article

Yi et al Nat Biotechnol. 2023 May 22. doi: 10.103

Insert

COX8-3xFlag-TALE(RNR2-Right)-TadA(8e+V106W)

Expression

Mammalian

Available Since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti NbALFA-mNeonGreen hygro

Plasmid

#193974

Purpose

lentivector coding for mNeonGreen fused to a nanobody targeting the ALFA tag, hygromycin resistance

Depositor

Raphael Gaudin

Article

Bare et al bioRxiv 2025.10.07.680694

Insert

NbALFA-mNeonGreen

Use

Lentiviral

Tags

mNeonGreen

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pJL1-SpCas9

Plasmid

#117051

Purpose

In vitro expression of S. pyogenes Cas9 from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al ACS Synth Biol. 2019 May 7. doi: 10.1021

Insert

S. pyogenes Cas9

Expression

Bacterial

Promoter

T7

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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mAmetrine-N1

Plasmid

#54505

Purpose

Localization: N1 Cloning Vector, Excitation: 406, Emission: 526

Depositor

Robert Campbell, Michael Davidson

Article

Ai et al Nat Methods. 2008 May;5(5):401-3. doi: 1

Type

Empty backbone

Tags

mAmetrine

Expression

Mammalian

Available Since

July 2, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pLEX305_FKBP12F36V-SHOC2

Plasmid

#134522

Purpose

Lentiviral plasmid expressing SHOC2 with N-term FKBP12(F36V) tag

Depositor

Andrew Aguirre

Article

Sulahian et al Cell Rep. 2019 Oct 1;29(1):118-134.e8. d

Insert

SHOC2 (SHOC2 Human)

Use

Lentiviral

Tags

FKBP12F36V-2xHA

Expression

Mammalian

Mutation

Silent mutations made at wobble base position at …

Promoter

hPGK

Available Since

Nov. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-fDIO mCherry

Plasmid

#114471

Purpose

Flp-dependent mCherry Control

Depositor

Karl Deisseroth

Has Service

AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

Insert

mCherry

Use

AAV

Promoter

Ef1a

Available Since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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CMV:AsCpf1-2A-GFP-U6-sgRNA-cloning vector

Plasmid

#194715

Purpose

Bicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that can be cloned with BbsI sites

Depositor

Rudolf Jaenisch

Article

Ma et al Bio Protoc. 2024 Nov 20;14(22):e5108. do

Insert

AsCpf1

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pAW016-2

Plasmid

#85613

Purpose

Cas9-tracrRNA integration vector for Bacillus subtilis

Depositor

Perry Chou

Article

Westbrook et al Appl Environ Microbiol. 2016 Jun 3. pii:

Insert

cas9-tracrRNA

Expression

Bacterial

Available Since

Jan. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pWPI-IRES-Bla-Ak

Plasmid

#154980

Purpose

Lentiviral vector for expressing mammalian proteins

Depositor

Sonja Best

Type

Empty backbone

Use

Lentiviral

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pET-45b-mEGFP-HMGB1-WT

Plasmid

#194543

Purpose

Bacterial Expression of 6xHis mEGFP-HMGB1 WT Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Tags

mEGFP

Expression

Bacterial

Available Since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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