167,150 results

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EFS-GFP

Plasmid

#110834

Purpose

positive control for GFP expression

Depositor

Michael Lewis

Article

Wei et al Stem Cells. 2014 Oct;32(10):2571-82. doi

Insert

elongation factor 1alpha binding sequence (EFS) at GFP promoter region

Use

Lentiviral

Expression

Mammalian

Available Since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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SGEP

Plasmid

#111170

Purpose

miR-E (miR-30 variant)-based RNAi

Depositor

Johannes Zuber

Article

Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

Insert

miR-E (miR-30 variant)

Use

Lentiviral

Mutation

WT

Promoter

SFFV

Available Since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-Kif5c-Myc

Plasmid

#186616

Purpose

Third generation lentiviral vector expressing Kinesin Heavy Chain 5 isoform C fused to Myc tag

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Kinesin Heavy Chain isoform 5c (KIF5C Human)

Use

Lentiviral

Tags

Myc tag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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V5-LOV-Turbo-ERM

Plasmid

#199664

Purpose

expresses LOV-Turbo on the mammalian ER membrane, lentiviral vector

Depositor

Alice Ting

Article

Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

Insert

LOV-Turbo

Use

Lentiviral

Tags

SEC61B and V5

Expression

Mammalian

Promoter

TRE3G

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pJMP1

Plasmid

#79873

Purpose

Bacillus subtilis dCas9 expression vector; integrates into lacA/ganA

Depositor

Carol Gross, Jason Peters, Stanley Qi

Article

Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

Insert

dCas9

Use

CRISPR

Expression

Bacterial

Mutation

D10A, H840A

Promoter

xylA

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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CMV-Tol2 transposase

Plasmid

#158774

Purpose

Expression of Tol2 transposase

Depositor

Hugo Snippert

Article

Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

Insert

Tol2 tranposase

Expression

Mammalian

Mutation

Tol2 codon usage frequency well suitable for muri…

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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iNos-eGFP

Plasmid

#207251

Purpose

Reporter for expression of eGFP under control of iNos promoter

Depositor

Michelle Farkas

Article

Mas-Rosario et al Front Oncol. 2023 Apr 5;13:1151384. doi:

Insert

iNos Promoter (Nos2 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCIP-AMPKa1_WT

Plasmid

#79010

Purpose

For mammalian expression of WT AMPK alpha1.

Depositor

Reuben Shaw

Article

Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

Insert

AMPK alpha 1 (PRKAA1 Human)

Expression

Mammalian

Promoter

pCAGGs

Available Since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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CoV2-M-IRES-E

Plasmid

#177938

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence) (E, M )

Expression

Mammalian

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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NFAT/AP-1 3x luciferase

Plasmid

#11783

Depositor

Anjana Rao

Article

Macian et al EMBO J. 2000 Sep 1. 19(17):4783-95.

Insert

NFAT/AP-1 3x (Il2 Mouse)

Use

Luciferase

Tags

luciferase

Expression

Bacterial

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pLG006 (Ec67 retron)

Plasmid

#157884

Purpose

Expresses the Ec67 retron defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

Ec67 retron

Expression

Bacterial

Available Since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-GAlphaQ-RLuc8

Plasmid

#140982

Purpose

Encodes a G alpha subunit (GNAQ) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

Depositor

Bryan Roth

Article

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Insert

GAlphaQ-RLuc8 (GNAQ Human)

Use

Luciferase

Expression

Mammalian

Mutation

RLuc8 and flanking SGGGGS linkers have been inser…

Promoter

CMV

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pET30b-5M SrtA with TEV site

Plasmid

#86962

Purpose

Expresses calcium-dependent Sortase A with a TEV cleavage site proximal to the C-terminal histidine tag

Depositor

Ron Bose

Article

Sarpong et al Anal Biochem. 2017 Mar 15;521:55-58. doi

Insert

S.aureus SrtA 5M

Tags

6x Histidine tag and TEV site

Expression

Bacterial

Mutation

P94R, D160N, D165A, K190E, K196T

Promoter

T7

Available Since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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mRFP-FKBP12

Plasmid

#67514

Purpose

FKBP12 only

Depositor

Tamas Balla

Article

Varnai et al J Cell Biol. 2006 Nov 6;175(3):377-82.

Insert

FKBP12 (FKBP1A Human)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 9, 2015

Availability

Academic Institutions and Nonprofits only

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TERT - WT

Plasmid

#213827

Purpose

Expresses human telomerase reverse transcriptase, the catalytic subunit of telomerase

Depositor

Coleman Lindsley

Article

Reilly et al Blood. 2021 Sep 9;138(10):898-911. doi:

Insert

human TERT (TERT Human)

Use

Lentiviral

Expression

Mammalian

Promoter

Tet-responsive promoter PTight, consisting of sev…

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-RhebL1

Plasmid

#133766

Purpose

Expresses GFP-tagged RhebL1 in mammalian cells

Depositor

Shawn Ferguson

Article

Angarola et al Mol Biol Cell. 2019 Oct 15;30(22):2750-2

Insert

RhebL1 (RHEBL1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pAD-sfGFP(Sp)-Pman

Plasmid

#133833

Purpose

Fluorescent protein sfGFP(Sp); under control of mannose inducible promoter.

Depositor

Oscar Kuipers

Article

Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57

Insert

sfGFP(Sp)

Use

E.coli/bacillus spp. shuttle vector

Expression

Bacterial

Promoter

Pman

Available Since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pZP06C

Plasmid

#232322

Purpose

pZP06C was constructed by adding the mCherry reporter gene driven by the rpsJ promoter into pZP06B (containing PrpsJ-sacB) to facilitate the screening of positive clones through red fluorescence.

Depositor

Chenggang Wu

Insert

mCherry

Expression

Bacterial

Promoter

rpsJ

Available Since

March 12, 2025

Availability

Academic Institutions and Nonprofits only

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plenti_DCLK1

Plasmid

#163625

Purpose

Expresses DCLK1

Depositor

Kenneth Westover

Article

Liu et al Cell Chem Biol. 2020 Oct 15;27(10):1229-

Insert

DCLK1 (DCLK1 Human)

Use

Lentiviral

Tags

V5

Promoter

sv40

Available Since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-LOV2

Plasmid

#81041

Purpose

Mammalian or insect expression for wt LOV2

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOV2

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV, p10

Available Since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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