174,421 results

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Artichoke

Plasmid

#73320

Purpose

Artichoke is a protein degradation reporter plasmid in a lentiviral backbone (SFFV.BsmBIClonesite.17AARigidLinker-EGFP.IRES.mCherry.cppt.EF1a.PuroR)

Depositor

Benjamin Ebert

Article

Slabicki et al Nature. 2020 Sep;585(7824):293-297. doi:

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

SFFV and EF1a

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA6 asyn WT

Plasmid

#107425

Purpose

Mammalian expression of human alpha synuclein

Depositor

Hilal Lashuel

Article

Mbefo et al J Biol Chem. 2015 Apr 10;290(15):9412-27

Insert

alpha-synuclein (SNCA Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pLentiPGK Puro DEST ERKKTRClover

Plasmid

#90227

Purpose

Lentiviral vector to express ERK KTR mClover under PGK promoter (With Puromycin Resistance)

Depositor

Markus Covert

Article

Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

Insert

ERK Kinase Translocation Reporter

Use

Lentiviral

Tags

mClover

Expression

Mammalian

Promoter

PGK

Available Since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-AviTag-SnoopLigase

Plasmid

#105626

Purpose

Plasmid for recombinant bacterial expression of SnoopLigase, a protein that promotes the formation of an isopeptide bond between 2 peptide tags (SnoopTagJr and DogTag), with AviTag for biotinylation

Depositor

Mark Howarth

Article

Buldun et al J Am Chem Soc. 2018 Feb 28;140(8):3008-3

Insert

AviTag-SnoopLigase

Expression

Bacterial

Promoter

T7

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pPBbsr2-4031NES

Plasmid

#105241

Purpose

A Förster resonance energy transfer (FRET) biosensor for AMPK activity with cyan and yellow fluorescent protein.

Depositor

Michiyuki Matsuda

Article

Konagaya et al Cell Rep. 2017 Nov 28;21(9):2628-2638. d

Insert

AMPK-EV

Expression

Mammalian

Promoter

CAGGS

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLAG-YY1

Plasmid

#104396

Purpose

FLAG-YY1

Depositor

Richard Young

Article

Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

Insert

YY1

Tags

FLAG

Available Since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pJK02

Plasmid

#105133

Purpose

Clostridium difficile CRISPR-cas9 mutagenesis plasmid traJ oriT

Depositor

Joseph Sorg

Article

McAllister et al Sci Rep. 2017 Nov 7;7(1):14672. doi: 10.

Inserts

Upstream & Downstream pyrE deletion region
tetR
Cas9
gRNA

Use

E. coli - c. difficile shuttle vector

Available Since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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mScarlet-I-mTurquoise2

Plasmid

#98839

Purpose

Produces a fusion between mTurquoise2 and mScarletI. It can be used as a positive control for FRET

Depositor

Dorus Gadella

Article

Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

Insert

mScarletI-mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pSF3-Flag-CBir-FRB_Myc-NBir-FKBP

Plasmid

#90003

Purpose

Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-FRB and N-terminally tagged NBir[2-256]-FKBP

Depositor

Julien Béthune

Article

Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

Inserts

NBirA*-linker-FKBP
CBirA*-linker-FRB

Use

Retroviral; Flp/frt

Tags

FLAG and Myc

Expression

Mammalian

Promoter

CMV

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1 (-) + FLAG-NLS-MS2-eGFP

Plasmid

#86827

Purpose

Expresses a nuclear localized, FLAG-tagged MS2-GFP fusion protein in mammalian cells, used for purification or localization of tagged RNAs

Depositor

Carl Novina

Article

Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

Insert

FLAG-NLS-MS2-eGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2017

Availability

Academic Institutions and Nonprofits only
pcDNA3-TDsmURFP-IRES-HO-1

Plasmid

#80346

Purpose

Expresses Tandem Dimer smURFP and Heme Oxygenase-1 in mammalian cells.

Depositor

Erik Rodriguez, Roger Tsien

Article

Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

Inserts

Tandem Dimer smURFP
HO-1

Expression

Mammalian

Promoter

CMV and IRES

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pLgw V5-EcoDam

Plasmid

#59210

Purpose

Mammalian DamID control lentiviral vector for expression of V5-Dam only

Depositor

Bas van Steensel

Article

Vogel et al Genome Res. 2006 Dec;16(12):1493-504. Ep

Insert

EcoDam

Use

Lentiviral; Damid

Tags

V5

Expression

Mammalian

Promoter

Heat Shock Minimal Promoter and heat shock minima…

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-tagBFP

Plasmid

#75160

Purpose

Derived from LentiCRISPR v1 but expresses tagBFP instead of puromycin resistance

Depositor

Beat Bornhauser

Article

McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

tagBFP

Expression

Mammalian

Available Since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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pMXspuro-GFP

Plasmid

#74203

Purpose

Retroviral control vector containing GFP insert

Depositor

Jayanta Debnath

Article

Kenific et al J Cell Biol. 2016 Feb 29;212(5):577-590.

Insert

GFP

Use

Retroviral

Expression

Mammalian

Available Since

April 25, 2016

Availability

Academic Institutions and Nonprofits only

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Orai1-GCaMP6f

Plasmid

#73564

Purpose

Green fluorescent reporter of Orai1-associated calcium influx

Depositor

Michael Cahalan

Article

Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

Insert

Orai1-GCaMP6f (ORAI1 Synthetic, Human)

Tags

GCaMP6f

Expression

Mammalian

Promoter

CMV Immediate Early

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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pT2/LTR7-GFP

Plasmid

#62541

Purpose

LTR7-driving reporter

Depositor

Zsuzsanna Izsvak

Article

Wang et al Nature. 2014 Dec 18;516(7531):405-9. doi

Insert

LTR7-GFP

Use

Sleeping beauty transposon

Expression

Mammalian

Promoter

LTR7

Available Since

Jan. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pCI SMN2

Plasmid

#72287

Purpose

SMN2 exon 7 splicing cassette

Depositor

Elliot Androphy

Article

Lorson et al Proc Natl Acad Sci U S A. 1999 May 25;96

Insert

SMN2 (SMN2 Human)

Expression

Mammalian

Mutation

contains exons 6-7-8 including introns, multiple …

Promoter

CMV

Available Since

Jan. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA GNSTM-3-Flag-10-Lamp2b-HA

Plasmid

#71293

Purpose

Encodes (N to C): GNSTM glycosylation motif, 3 residue spacer, Flag tag,10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag.

Depositor

Joshua Leonard

Article

Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72

Insert

Lamp2b (LAMP2 Human)

Tags

FLAG, GNSTM glycosylation motif, HA, and Lamp2 si…

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pYPQ150

Plasmid

#69301

Purpose

Gateway entry vector with pcoCas9 (Plant codon optimized)

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

pcoCas9 (plant codon-optimized)

Use

CRISPR

Tags

3XFLAG

Mutation

NLS fusion

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pDEST47-ARL8B-GFP

Plasmid

#67404

Purpose

Mammalian expression of hArl8b with C-term GFP tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL8B (ARL8B Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

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