161,547 results

1. pAAV-FLEX-SaCas9-U6-sgSlc17a6

   Plasmid

   #124847

   Purpose

   Mutagenesis of Slc17a6 with SauCas9

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Slc17a6 gRNA (Slc17a6 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR223_LCK_WT

   Plasmid

   #82305

   Purpose

   Gateway Donor vector containing LCK, part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, Jesse Boehm, William Hahn

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   LCK (LCK Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   V49I

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-LMO-IRES-EGFP

   Plasmid

   #178309

   Purpose

   Express the bacterial lactate 2-monooxygenase (LMO) enzyme in mammalian cells

   Depositor

   Sergey Kasparov

   Article

   Vaccari Cardoso et al Brain Sci. 2021 Aug 10;11(8). pii: brain

   Insert

   Lactate 2-monooxygenase
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. mRuby2-Calreticulin-N-16

   Plasmid

   #55892

   Purpose

   Localization: Endoplasmic Reticulum, Excitation: 559, Emission: 600

   Depositor

   Michael Davidson

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Insert

   Calreticulin (CALR Human)
   

   Use

   Tags

   mRuby2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 10, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

5. pGD-p19

   Plasmid

   #196326

   Purpose

   35S promoter-driven expression of the tomato bushy stunt virus p19 RNA silencing suppressor

   Depositor

   Zhenghe Li

   Article

   Ganesan et al J Virol. 2013 Oct;87(19):10598-611. doi:

   Insert

   tomato bushy stunt virus p19
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   duplicated cauliflower mosaic virus 35S promoter

   Available since

   May 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. CCT8_pCSdest

   Plasmid

   #53795

   Depositor

   Roger Reeves

   Article

   Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

   Insert

   CCT8 (CCT8 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SP6 and CMV

   Available since

   Sept. 9, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

7. pUC18R6K-mini-Tn7T-Gm

   Plasmid

   #65022

   Purpose

   GmR mini-Tn7 vector, R6K replicon

   Depositor

   Herbert Schweizer

   Article

   Choi et al Nat Protoc. 2006;1(1):153-61.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 17, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pD649-HAsp-TNFSF14-COMP5AP-AviTag-9xHis

   Plasmid

   #157149

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   TNFSF14 (TNFSF14 Human)
   

   Use

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/SP6

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. BirA(R118G)-HA destination vector

   Plasmid

   #53581

   Purpose

   To fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID)

   Depositor

   Karl Kramer

   Type

   Empty backbone

   Use

   Tags

   BirA(R118G) and HA tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. FUW TetO HA-AMPKa1 WT

    Plasmid

    #69824

    Purpose

    Expresses AMPK alpha 1 in mammalian cells in a doxycycline inducible manner

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    minimal CMV promoter with tetracycline operator

    Available since

    April 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pIR-CMV-SCN1A-Variant-1-IRES-mScarlet

    Plasmid

    #162278

    Purpose

    Eukaryotic expression of human SCN1A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.

    Depositor

    Al George

    Article

    DeKeyser et al J Biol Chem. 2021 Jan-Jun;296:100298. do

    Insert

    SCN1A Variant 1, stabilized (SCN1A Human)
    

    Use

    Tags

    IRES-mScarlet

    Expression

    Mammalian

    Mutation

    IVS intron inserted after bp2946. Silent codon c…

    Promoter

    CMV

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. MSCV-hCD19

    Plasmid

    #127889

    Purpose

    Expresses hCD19

    Depositor

    Anjana Rao

    Article

    Chen et al Nature. 2019 Mar;567(7749):530-534. doi:

    Insert

    hCD19 (CD19 Human)
    

    Use

    Retroviral

    Tags

    no tags

    Expression

    Mutation

    none

    Promoter

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSLQ10844

    Plasmid

    #183956

    Purpose

    CAG-hyperdCas12a-HA-miniVPR-mCherry-T2A-HygroR

    Depositor

    Stanley Qi

    Article

    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

    Insert

    HyperdCas12a
    

    Use

    CRISPR

    Tags

    HA, Hygromycin Resistance, and mCherry

    Expression

    Mammalian

    Mutation

    D832A, D156R, E292R, D235R, D350R

    Promoter

    CAG

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. TFORF3198

    Plasmid

    #144674

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    OVOL2 (OVOL2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. mCherry-INPP5K

    Plasmid

    #171950

    Purpose

    Expresses INPP5K in mammalian cells

    Depositor

    Cheng-han Yu

    Article

    Feng et al Proc Natl Acad Sci U S A. 2021 May 11;11

    Insert

    INPP5K (INPP5K Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. NET33 pEGFP-N2 (597)

    Plasmid

    #61989

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

    Insert

    NET33 (SCARA5 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAVtri-CMV-eGFP

    Plasmid

    #230932

    Purpose

    Used for producing AAV-CMV-eGFP viruses with the AAVtri triple-plasmid system. This system combines the use of the mini-pHelper-1.0 and AAV helper plasmids carrying different AAV serotypes.

    Depositor

    Qizhao Wang

    Insert

    eGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEL066

    Plasmid

    #124897

    Purpose

    For genome editing in plant by SpCas9 variant(SpCas9-NG) (D10A)-PmCDA1-UGI base editor

    Depositor

    Yong Zhang

    Article

    Zhong et al Mol Plant. 2019 Mar 27. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    3xFlag

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. kiCAP-AAV-BI-hTFR1

    Plasmid

    #218796

    Purpose

    RepCap for AAV production

    Depositor

    Ben Deverman

    Article

    Huang et al Science. 2024 Jun 14;384(6701):1220-1227

    Insert

    AAV Rep-Cap BI-hTFR1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. dCas9-GCN5 FL

    Plasmid

    #179552

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of full length human GCN5 driven by EF-1alpha promoter

    Depositor

    Isaac Hilton

    Article

    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

    Insert

    S. pyogenes dCas9 with c-terminal fusion of full length human GCN5 (KAT2A Human)
    

    Use

    Lentiviral

    Tags

    Flag Tag

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    EF-1alpha

    Available since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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