179,813 results
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Plasmid#137880PurposeDonor vector for integration at the human AAVS1 safe harbor locus of Doxicycline-inducible dCas9-VPR-T2A-EGFPDepositorInsertTRE3G-dCas9-VPR-T2A-EGFP-CAG-TetON-NeoR-SA
UseAAVExpressionMammalianAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pC0079 RESCUE-S
Plasmid#130662PurposepCMV-dCas13b6-mapkNES-GS-dADAR2(E488Q/V351G/S486A/T375A/S370C/P462A/N597I/L332I/I398V/K350I/M383L/D619G/S582T/V440I/S495N/K418E/S661T) V16SDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdRanCas13b-ADAR2dd
ExpressionMammalianAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Mito-mEosEM
Plasmid#132706Purposeapplication in Correlative light-electron microscopyDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmitochondria targeting sequence
TagsmEosEMExpressionMammalianPromoterCMVAvailable sinceOct. 4, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSH-EFIRES-P-AtAFB2
Plasmid#129715PurposeAAVS1 safe harbor targeting vector expressing AtAFB2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAFB2 (AFB2 Mustard Weed)
UseCRISPR and TALEN; Human safe harbor locus (a avs1…TagsnoExpressionMammalianMutationcodon optimizedPromoterEF1aAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
TEVSH
Plasmid#125194PurposeHis-tagged TEV protease with solubility increasing mutationsDepositorInsertTEV_SH
TagsV5 His-tagExpressionBacterialPromoterT7lacAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mCherry- SEC61B
Plasmid#121160PurposeMammalian expression of mCherry tagged SEC61B, used as a general ER markerDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSEC61B (SEC61B )
ExpressionMammalianAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHR OVA/p2a/mCherry-CaaX
Plasmid#113030Purposefor lentivirus production of ovalbumin and mcherry-caaxDepositorInsertOVA/p2a/mCherry-CaaX
UseLentiviralTagsmCherryExpressionMammalianAvailable sinceSept. 12, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-GRAB_DA1h
Plasmid#113050Purposeexpress the genetically-encoded fluorescent dopamine(DA) sensor GRAB_DA1h in neuronsDepositorHas serviceAAV9 and AAV RetrogradeInsertGPCR activation based DA sensor GRAB_DA1h (DRD2 Human)
UseAAVPromoterhSynAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKC-4.06
Plasmid#112084PurposeNMD sensor: pCMV-3XFLAG-Fluc-betaglobin (39PTC)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert3XFLAG firefly luciferase-beta-globin 39PTC (HBB Photinus pyralis, Mouse, Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Artichoke
Plasmid#73320PurposeArtichoke is a protein degradation reporter plasmid in a lentiviral backbone (SFFV.BsmBIClonesite.17AARigidLinker-EGFP.IRES.mCherry.cppt.EF1a.PuroR)DepositorTypeEmpty backboneUseLentiviralTagsEGFPExpressionMammalianPromoterSFFV and EF1aAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CWB-yellow pre-eGRASP(p32)
Plasmid#111580PurposeAn AAV vector that expresses yellow pre-eGRASP(p32) under the CaMKIIa promoter.DepositorInsertyellow pre-eGRASP(p32)
UseAAVPromoterCaMKIIaAvailable sinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pPBbsr2-4031NES
Plasmid#105241PurposeA Förster resonance energy transfer (FRET) biosensor for AMPK activity with cyan and yellow fluorescent protein.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAMPK-EV
ExpressionMammalianPromoterCAGGSAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1 FLAG-YY1
Plasmid#104396PurposeFLAG-YY1DepositorInsertYY1
TagsFLAGAvailable sinceFeb. 5, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pC0039-CMV-dPspCas13b-GS-ADAR2DD(E488Q)
Plasmid#103849PurposedPspCas13b-ADAR2DD(E488Q) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsUseCRISPRTagsGS and HIV NESExpressionMammalianMutationChanged histidne 133 to alanine and histidine 105…PromoterCMVAvailable sinceNov. 28, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Tau (wt) -VC
Plasmid#87369Purposeexpresses hTau fused to the C-terminal part of VenusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMAPT (MAPT Human)
Tagsc-terminal part of VenusExpressionMammalianPromoterCMVAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
U6REST1_U6REST2.hPGK.BRN2.hPGK.Ascl1WPRE
Plasmid#101852PurposeDirect reprogramming of adult fibroblasts to neuronsDepositorInsertAscl1, Brn2
UseLentiviralPromoterU6, PGKAvailable sinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mScarlet-I-mTurquoise2
Plasmid#98839PurposeProduces a fusion between mTurquoise2 and mScarletI. It can be used as a positive control for FRETDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmScarletI-mTurquoise2
ExpressionMammalianPromoterCMVAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
htrpC6-YFP
Plasmid#21084DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTrpC6 (TRPC6 Human)
TagsYFPExpressionMammalianAvailable sinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCDNA3_Sec61B-V5-APEX2
Plasmid#83411PurposeExpressed C-term tagged APEX2 on Sec61B in mammalian cellDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSec61B-V5-APEX2 (SEC61B Human, Synthetic)
TagsAPEX2 and V5ExpressionMammalianPromoterCMV-FAvailable sinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHW394 (15xUAS::GFP::let-858 3'UTR)
Plasmid#85584PurposecGAL effector (15xUAS::GFP::let-858 3'UTR) construct for C. elegansDepositorInsert15xUAS-delta pes-10-GFP
ExpressionWormMutationGFP(S65C) with three introns for expression in C.…Promoter15xUAS-delta pes-10-GFPAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by