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Characterization of a spectrally diverse set of fluorescent proteins as FRET acceptors for mTurquoise2.
Mastop M, Bindels DS, Shaner NC, Postma M, Gadella TWJ Jr, Goedhart J
Sci Rep. 2017 Sep 20;7(1):11999. doi: 10.1038/s41598-017-12212-x.
PubMed

Plasmids from Article

ID Plasmid Purpose  
98835Gq-sensor[mTq2-sREACh]A FRET sensor for Gq activation, encoded on a single plasmid, for FLIM
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98837mKOkappa-2A-mTurquoise2Produces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRET
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98838mKO2-2A-mTurquoise2Produces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRET
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98839mScarlet-I-mTurquoise2Produces a fusion between mTurquoise2 and mScarletI. It can be used as a positive control for FRET
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98840sReach-mTurquoise2Produces a fusion between mTurquoise2 and sReach. It can be used as a positive control for FRET (using FLIM)
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98884Gq-sensor[mTq2-mNG]A FRET sensor for Gq activation, encoded on a single plasmid.
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98885mNeonGreen-2A-mTurquoise2Produces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRET
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98886mNeonGreen-mTurquoise2Produces a fusion between mTurquoise2 and mNeonGreen. It can be used as a positive control for FRET
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