163,584 results

1. pCAG-enAsCas12a(E174R/S542R/K548R)-NLS(nuc)-3xHA (AAS848)

   Plasmid

   #107941

   Purpose

   Mammalian expression plasmid for human codon optimized enAsCas12a (enhanced AsCas12a) encoding E174R/S542R/K548R substitutions

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized enAsCas12a (E174R/S542R/K548R)
   

   Use

   Tags

   3x HA and NLS (nucleoplasmin)

   Expression

   Mammalian

   Mutation

   E174R, S542R and K548R

   Promoter

   CAG

   Available Since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. plentiCMV-sct-pp65-b2m-HLA-A0201

   Plasmid

   #196511

   Purpose

   Express single-chain peptide-MHC (pp65 CMV epitope on HLA-A0201)

   Depositor

   Howard Chang

   Article

   Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

   Insert

   single chain of a pp65 peptide, b2m and HLA A0201
   

   Use

   Lentiviral

   Tags

   NA

   Expression

   Mammalian

   Mutation

   Y84C

   Promoter

   CMV

   Available Since

   March 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSBbi-Pur-dCas9-KRAB-MeCP2_hU6-SapI

   Plasmid

   #196078

   Purpose

   (Empty Backbone) Constitutive CRISPRi vector conferring puromycin resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.

   Depositor

   Joanna Bereta

   Article

   Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

   Inserts

   dCas9-KRAB-MeCP2 repressor
   hU6 promoter and gRNA scaffold
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Catalytically dead mutant of the Cas9 endonucleas…

   Promoter

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLifeact-mScarlet3-H (mYongHong)

   Plasmid

   #239415

   Purpose

   Expresses Lifeact-mScarlet3-H (aka mYongHong) in mammalian cells

   Depositor

   Zhifei Fu

   Article

   Xiong et al Nat Methods. 2025 Apr 17. doi: 10.1038/s

   Insert

   Lifeact-mScarlet3-H
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   M163H in mScarlet3

   Promoter

   CMV

   Available Since

   Aug. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCINEO-3xFlag-hRIPK3

   Plasmid

   #112495

   Purpose

   Mammalian expression of 3xFlag-hRIPK3

   Depositor

   Junying Yuan

   Insert

   RIPK3 (RIPK3 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. Cytoplasmic cpGFP

   Plasmid

   #215709

   Purpose

   Expresses cpGFP in the cytoplasm

   Depositor

   Katharine Diehl

   Article

   Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

   Insert

   cpGFP
   

   Use

   Lentiviral

   Tags

   FLAG, HA, and cytoplasmic localization sequence

   Expression

   Mammalian

   Mutation

   circularly permuted GFP

   Promoter

   CMV

   Available Since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA-Cas9-T2A-TdT

   Plasmid

   #126424

   Purpose

   Expresses Cas9-T2A-TdT in mammalian cells. (pTBL209)

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Insert

   Cas9-T2A-TdT (DNTT Streptococcus pyogenes, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV

   Available Since

   July 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. OKSIM

   Plasmid

   #24603

   Depositor

   Jose Cibelli

   Article

   Ross et al Stem Cells Dev. 2010 Aug;19(8):1221-9

   Insert

   Oct4 KLF4 Sox2 c-Myc (SOX2, POU5F1, MYC, KLF4 Human)
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Oct4-2A-KLF4-2A-Sox2-IRES-Myc

   Promoter

   Available Since

   June 29, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV_CKAR3

   Plasmid

   #238413

   Purpose

   AAV construct with CKAR3 expression under a human synapsin promotor and WPRE3 translation enhancer.

   Depositor

   Haining Zhong

   Article

   Yahiro et al bioRxiv [Preprint]. 2024 Jul 23:2024.07.

   Insert

   CKAR3 (PKC sensor)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLG010 (DRT type 2)

    Plasmid

    #157888

    Purpose

    Expresses the DRT type 2 defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    DRT type 2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-hSyn-ChRmine-mScarlet-WPRE

    Plasmid

    #130994

    Purpose

    AAV vector to drive the expression of ChRmine-mScarlet under the control of human synapsin promoter

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Insert

    ChRmine
    

    Use

    AAV

    Tags

    mScarlet

    Expression

    Mutation

    Promoter

    hSyn

    Available Since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. Lamp1-mTurquoise2

    Plasmid

    #98828

    Purpose

    In vivo visualization of lysosomes (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    Lamp1
    

    Use

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSG5-BZLF1

    Plasmid

    #72637

    Purpose

    Mammalian expression vector for Epstein-Barr virus Zta immediate-early transcriptional regulator

    Depositor

    S. Diane Hayward

    Article

    Sarisky et al J Virol. 1996 Dec;70(12):8340-7.

    Insert

    BZLF1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q163R

    Promoter

    Available Since

    Feb. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA-K-GECO1

    Plasmid

    #105865

    Purpose

    Mammalian cell expression plasmid for red fluorescent Ca2+ indicator K-GECO1

    Depositor

    Robert Campbell

    Article

    Shen et al BMC Biol. 2018 Jan 16;16(1):9. doi: 10.1

    Insert

    K-GECO1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG_PSD95.FingR-eGFP-CCR5TC

    Plasmid

    #46295

    Depositor

    Don Arnold

    Article

    Gross et al Neuron. 2013 Jun 19;78(6):971-85. doi: 1

    Insert

    PSD95.FingR-eGFP-CCR5TC (DLG4 Human, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    July 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hChATp-tdTomato

    Plasmid

    #197564

    Purpose

    Expresses tdTomato under a human choline acetyltransferase (hChAT) promoter fragment

    Depositor

    Casey Maguire

    Article

    Santoscoy et al Mol Ther Methods Clin Dev. 2023 May 9;29

    Insert

    tdTomato
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    human ChAT promoter

    Available Since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. PGK-H2BeGFP

    Plasmid

    #21210

    Depositor

    Mark Mercola

    Article

    Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

    Insert

    Phosphoglycerate kinase promoter (PGK1 Human)
    

    Use

    Lentiviral

    Tags

    H2B-eGFP

    Expression

    Mutation

    Promoter

    Available Since

    July 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDY0863 PASTEv4 pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT

    Plasmid

    #179106

    Purpose

    pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pENTR-ABCB1_STOP

    Plasmid

    #221424

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Wolf et al Cell Chem Biol. 2024 Dec 24:S2451-9456(2

    Insert

    ABCB1 (ABCB1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCM184

    Plasmid

    #46012

    Depositor

    Mary Lidstrom

    Article

    Marx et al Biotechniques. 2002 Nov;33(5):1062-7.

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    na

    Available Since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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