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hSyn1-sypHy-RGECO
(Plasmid #84078)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 84078 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-N2
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 3903
  • Total vector size (bp) 6846
  • Modifications to backbone
    CMV promoter replaced with hSyn1 promoter, EGFP removed.
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sypHy-RGECO
  • Alt name
    Rat Synaptophysin-pHluorin and R-GECO1
  • Species
    R. norvegicus (rat), Synthetic
  • Insert Size (bp)
    2943
  • Promoter hSyn1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer ACTCAGCGCTGCCTCAGTC
  • 3′ sequencing primer TTATGTTTCAGGTTCAGGG
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    SypHy was subcloned from CMV-SypHy A4 (Addgene 24478) and R-GECO1 from CMV-R-GECO1 (Addgene 32444)
  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hSyn1-sypHy-RGECO was a gift from Juan Burrone (Addgene plasmid # 84078 ; http://n2t.net/addgene:84078 ; RRID:Addgene_84078)
  • For your References section:

    Visualizing Presynaptic Calcium Dynamics and Vesicle Fusion with a Single Genetically Encoded Reporter at Individual Synapses. Jackson RE, Burrone J. Front Synaptic Neurosci. 2016 Jul 26;8:21. doi: 10.3389/fnsyn.2016.00021. eCollection 2016. 10.3389/fnsyn.2016.00021 PubMed 27507942