163,445 results

1. pHAGE-PTEN

   Plasmid

   #116780

   Purpose

   Lentiviral expression of PTEN

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   PTEN (PTEN Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLOX-TERT-iresTK

   Plasmid

   #12245

   Purpose

   2nd generation lentiviral vector for reversible immortalization of human primary cells

   Depositor

   Didier Trono

   Article

   Salmon et al Mol Ther. 2000 Oct . 2(4):404-14.

   Insert

   TERT, HSV1-TK (TERT Human)
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   LoxP in 3' LTR.

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato]

   Plasmid

   #62723

   Purpose

   AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. TdTomato is a codon diversified version.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Has Service

   AAV1 and AAV5

   Insert

   ChrimsonR-tdTomato
   

   Use

   AAV

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Chrimson K176R mutant

   Promoter

   human synapsin promoter

   Available since

   April 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pKrox24(2xD-E_inD)Luc

   Plasmid

   #200109

   Purpose

   Luciferase reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   modified EGR1 promoter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   highly modified sequence

   Promoter

   2 copies of D-E element in front of EGR1 promoter…

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. mTq2-Calmodulin

   Plasmid

   #198200

   Purpose

   FRET donor: Calmodulin-2 tagged with N-terminal mTurquoise2

   Depositor

   Christian Wahl-Schott

   Article

   Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

   Insert

   Calmodulin 2 (Calm2 Rat)
   

   Use

   Tags

   mTurquoise2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHH0103_GNAI2_G-alpha

   Plasmid

   #109791

   Purpose

   Protein expression and purification of GNAI2_G-alpha

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   GNAI2_G-alpha (GNAI2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pD21-AI-hLDLR

   Plasmid

   #124964

   Purpose

   Express human LDLR under human APOAI promoter

   Depositor

   Kazuhiro Oka

   Article

   Li et al J Genet Syndr Gene Ther. 2011 Sep 28;2:1

   Insert

   human LDLR (LDLR Human)
   

   Use

   Adenoviral

   Tags

   Expression

   Mutation

   Promoter

   human APOAI

   Available since

   Nov. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AAVS1-Bxb1-LP-TC

   Plasmid

   #183754

   Purpose

   AAVS1 targeting construct with the Bxb1 landing pad cassette.

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

   Insert

   loxP-eGFP-attP-Bsd-lox257
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pIB185

   Plasmid

   #90196

   Purpose

   E. coli - Streptococci shuttle plasmid for gene expression in streptococci with Pveg promoter

   Depositor

   Indranil Biswas

   Article

   Biswas et al Microbiology. 2008 Aug;154(Pt 8):2275-82

   Type

   Empty backbone

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV.hSyn.FLEX.iGluSnFR3.v82.GPI.codonopt

    Plasmid

    #175184

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 82 in GPI backbone. iGluSnFR3.v82.GPI

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    pAAV hSyn FLEX iGluSnFR3 v82.GPI
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    GPI Anchor, IgK-chain, and Myc epi tag

    Expression

    Mammalian

    Mutation

    Promoter

    human Synapsin

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBR_attB(bxb)_ccdB_lox_v2

    Plasmid

    #198040

    Purpose

    Bxb1-specific donor plasmid for the cloning of multiple inserts by Golden Gate assembly (BpiI)

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Nat Protoc. 2024 Aug 23. doi: 10.1038/s4

    Type

    Empty backbone

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. SOST/pcDNA3.1+

    Plasmid

    #10842

    Depositor

    Xi He

    Article

    Semenov et al J Biol Chem. 2005 Jul 22. 280(29):26770-

    Insert

    SOST (SOST Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCIN BRCA2 WT

    Plasmid

    #16245

    Depositor

    Mien-Chie Hung

    Article

    Wang et al Cancer Res. 2002 Mar 1. 62(5):1311-4.

    Insert

    BRCA2 (BRCA2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. TCARZ-CMV-Cherry-PAmin-GF3-R4A22

    Plasmid

    #203770

    Purpose

    For production of template RNA for use in PRINT, mCherry under CMV promoter

    Depositor

    Kathleen Collins

    Article

    Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

    Insert

    PRINT template RNA for mCherry
    

    Use

    Ivt template

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCpfSA

    Plasmid

    #187890

    Purpose

    CRISPR/Cpf1-based Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus

    Depositor

    Quanjiang Ji

    Article

    Wang et al ACS Synth Biol. 2022 Sep 16;11(9):3049-3

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLVX-Puro-TDP-43-Q331K

    Plasmid

    #133761

    Purpose

    Lentiviral expression of human TDP-43 Q331K disease mutant in mammalian cells.

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Changed glutamine 331 to lysine.

    Promoter

    CMV

    Available since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. FUW-TetON-Sox10

    Plasmid

    #115242

    Purpose

    Lentiviral plasmid for tet-inducible expression of Sox10 (generated from plasmid #20321)

    Depositor

    Catherine Verfaillie

    Article

    Garcia-Leon et al Stem Cell Reports. 2018 Feb 13;10(2):655

    Insert

    SOX10 (SOX10 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLNCX2-mCherry-CHMP4B

    Plasmid

    #116923

    Purpose

    expresses mCherry-CHMP4B in mammalian cells

    Depositor

    Sanford Simon

    Article

    Bleck et al Proc Natl Acad Sci U S A. 2014 Aug 19;11

    Insert

    CHMP4B (CHMP4B Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti-PGK-Puro-TK-NLS-mTurq2-PCNA

    Plasmid

    #118617

    Purpose

    Lentiviral expression of mTurq2 tagged PCNA

    Depositor

    Jean Cook

    Article

    Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

    Insert

    TK-NLS-mTurq2-PCNA (PCNA Human)
    

    Use

    Lentiviral

    Tags

    mTurq2

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK

    Available since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenLox_U6 p53

    Plasmid

    #59360

    Purpose

    Lentiviral expression vector: p53 shRNA under control of mouse U6 promoter, EGFP-expression driven by CAG promoter to monitor expression.

    Depositor

    Peter Scheiffele

    Article

    Chih et al Science. 2005 Feb 25;307(5713):1324-8. E

    Inserts

    shRNA p53 (mouse) (Trp53 Mouse)
    eGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and mouse U6

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge