165,550 results

1. pCMV6-G1-ATP8-Myc-FLAG (G418)

   Plasmid

   #86850

   Purpose

   mammalian expression of ATP8 with mitochondrial targeting sequence, Myc, and FLAG tags

   Depositor

   Matthew O'Connor

   Article

   Boominathan et al Nucleic Acids Res. 2016 Nov 2;44(19):934

   Insert

   ATP8 (ATP8 Human)
   

   Tags

   ATP5G1 (G1) (MQTAGALFISPALIRCCTRGLIRPVSASFLNS PVN…

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   CMV

   Available Since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pgLAP4

   Plasmid

   #19705

   Depositor

   Peter Jackson

   Insert

   pgLAP4
   

   Tags

   Flag, S peptide, and Tev

   Expression

   Mammalian

   Available Since

   April 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. ER-B

   Plasmid

   #209870

   Purpose

   Dimerization dependent fluorescent protein B anchored to cytosolic face of the endoplasmic reticulum membrane with N-terminal targeting sequence of rabbit CYP2C1

   Depositor

   Sarah Cohen

   Article

   Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

   Insert

   ddGFP B
   

   Tags

   Targeting domain of CYP2C1 MDPVVVLGLCLSCLLLLSLWKQ…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEGFP-N1-MITF-M

   Plasmid

   #38131

   Depositor

   Shawn Ferguson

   Article

   Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

   Insert

   MITF-M (MITF Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   deleted stop codon

   Promoter

   CMV

   Available Since

   July 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. CMVTO-SEAP-26Sfur-3TM-tevs-AAMP

   Plasmid

   #179633

   Purpose

   SEAP RELEASE construct with AAMP motif control

   Depositor

   Xiaojing Gao

   Article

   Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

   Insert

   SEAP RELEASE
   

   Expression

   Mammalian

   Available Since

   March 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pH2B-Electra2

   Plasmid

   #179479

   Purpose

   Nuclear labeling with blue fluorescent protein Electra2 fused to H2B

   Depositor

   Kiryl Piatkevich

   Article

   Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

   Insert

   H2B-Electra2
   

   Tags

   Electra2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-ihSyn1-tTA

   Plasmid

   #99120

   Purpose

   An AAV genome that expresses tTA from the hSyn1 promoter that contains a positive feedback loop for amplifed expression of the tTA

   Depositor

   Viviana Gradinaru

   Article

   Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

   Has Service

   AAV1

   Insert

   tTA
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   *(see below)

   Promoter

   ihSyn1

   Available Since

   Aug. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. TFORF1842

   Plasmid

   #143324

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   YWHAZ (YWHAZ Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDONR223-TLK2

   Plasmid

   #23629

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   TLK2 (TLK2 Human)
   

   Use

   Gateway donor vector

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

10. TLCV2-RB1

    Plasmid

    #87836

    Purpose

    LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive expression of an sgRNA targeting human RB1.

    Depositor

    Adam Karpf

    Article

    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

    Insert

    sgRB1 (RB1 Human)
    

    Use

    CRISPR and Lentiviral; Doxycycline inducible; egf…

    Expression

    Mammalian

    Promoter

    Tight TRE promoter and U6

    Available Since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMBP-FnCas12a

    Plasmid

    #113432

    Purpose

    Expresses 10xHis-MBP fused to FnCas12a

    Depositor

    Jennifer Doudna

    Article

    Chen et al Science. 2018 Apr 27;360(6387):436-439.

    Insert

    FnCas12a
    

    Tags

    MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET31b-T7-Spinach2-Ct

    Plasmid

    #79158

    Purpose

    Expresses the Ct biosensor under a T7 promoter

    Depositor

    Ming Hammond

    Article

    Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

    Insert

    T7-tRNA-Spinach2-Ct
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDisplay-GRAB_SST1.0

    Plasmid

    #208664

    Purpose

    Expresses the genetically-encoded fluorescent somatostatin (SST) sensor GRAB_SST1.0 in mammalian cells

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based somatostatin (SST) sensor GRAB_SST1.0
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pENTR5'_ubi

    Plasmid

    #27320

    Depositor

    Leonard Zon

    Article

    Mosimann et al Development. 2011 Jan . 138(1):169-77.

    Insert

    zebrafish ubiquitin promoter (ubb Zebrafish)
    

    Use

    Multisite gateway 5' entry vector

    Available Since

    Feb. 3, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CpxR-GFP

    Plasmid

    #220150

    Purpose

    To track the natural CpxR promoter's transcriptional dynamics

    Depositor

    Andre Sanches Ribeiro

    Article

    Dash et al mSystems. 2024 Jun 18;9(6):e0006524. doi

    Insert

    CpxR promoter only
    

    Expression

    Bacterial

    Available Since

    Aug. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1-HaloAKAR2.1-T2A-EGFP

    Plasmid

    #216489

    Purpose

    Expression of chemigenetic PKA biosensor (medium basal brightness, high dynamic range) and EGFP transfection marker in mammalian cells

    Depositor

    Jin Zhang

    Article

    Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

    Insert

    HaloAKAR2.1
    

    Tags

    T2A-EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. lentiCRISPR v2-Nr4a1 double gRNAs

    Plasmid

    #162791

    Purpose

    Multiplex Guide RNAs to generate Nr4a1 knockout by CRISPR

    Depositor

    Matthew Steinhauser

    Article

    Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

    Insert

    Multiplex Nr4a1 gRNA 1 and 2 (Nr4a1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Promoter

    hU6

    Available Since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-S5E2-GFP-fGFP

    Plasmid

    #135631

    Purpose

    AAV vector to drive the expression of fGFP in PV cortical interneuronsunder the control of the E2 regulatory element

    Depositor

    Jordane Dimidschstein

    Article

    Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

    Has Service

    AAV PHP.eB, AAV1, and AAV9

    Insert

    GFP-fGFP
    

    Use

    AAV

    Mutation

    None

    Available Since

    Aug. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKm

    Plasmid

    #170331

    Purpose

    backbone vector for clone construction

    Depositor

    Deqiang Duanmu

    Article

    Wang et al Front Microbiol. 2021 Jun 18;12:686008.

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mEmerald-Talin-C-18

    Plasmid

    #62763

    Purpose

    Localization: Talin/Focal Adhesions, C1 Cloning Vector, Excitation:487, Emission:509

    Depositor

    Michael Davidson

    Insert

    Talin
    

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    March 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge