165,537 results

1. MSCV-Cas9-2A-BFP

   Plasmid

   #164662

   Purpose

   Retroviral introduction of Cas9 into mammalian cell line coupled to expression of blue fluorescent protein

   Depositor

   Daniel Hodson

   Article

   Caeser et al Nat Commun. 2019 Oct 4;10(1):4543. doi:

   Inserts

   Cas9
   BFP
   

   Use

   CRISPR and Retroviral

   Expression

   Mammalian

   Promoter

   MSSV_LTR

   Available Since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3 Connexin43-GFP-APEX2

   Plasmid

   #49385

   Purpose

   Expresses a tandem GFP-APEX2 fusion to the C-terminus of connexin43 in mammalian cells

   Depositor

   Alice Ting

   Article

   Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

   Insert

   Connexin43 - EGFP - APEX2
   

   Expression

   Mammalian

   Mutation

   K14D, W41F, E112K, A134P on soybean APX; improved…

   Promoter

   CMV

   Available Since

   Nov. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCOLA-T7-WspR

   Plasmid

   #79162

   Purpose

   Expresses WspR WT under a T7 promoter

   Depositor

   Ming Hammond

   Article

   Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

   Insert

   WspR (wspR P. areuginosa)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. hFerritinH

   Plasmid

   #122652

   Purpose

   Expression of hFerritinH in bacterial cells

   Depositor

   Trevor Douglas

   Insert

   ferritin heavy chain 1 (FTH1 Human)
   

   Expression

   Bacterial

   Available Since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. FUW-Sox10

   Plasmid

   #36978

   Depositor

   Bob Weinberg

   Article

   Guo et al Cell. 2012 Mar 2;148(5):1015-28.

   Insert

   Sox10 (SOX10 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   July 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLX311 Luciferase

   Plasmid

   #117735

   Purpose

   Open reading frame vector encoding Luciferase

   Depositor

   William Hahn

   Article

   Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

   Insert

   firefly Luciferase
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. dGFP-Rab3a

   Plasmid

   #170864

   Purpose

   Mammalian expression of dGFP-Rab3a.

   Depositor

   Justin Taraska

   Article

   Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

   Insert

   Rab-3a (Rab3a Mouse)
   

   Tags

   dEGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCSC-ISL1-T2A-LHX3

   Plasmid

   #90215

   Purpose

   To convert human skin fibroblasts into induced motor neurons (hiMN) in combination with NGN2, Sox11, FGF2 and two small molecules, forskolin and dorsomorphin.

   Depositor

   Chun-Li Zhang

   Article

   Liu et al Cell Rep. 2016 Jan 5;14(1):115-28. doi:

   Insert

   ISL1-T2A-LHX3 (ISL1, LHX3 Human)
   

   Use

   Lentiviral

   Promoter

   CMV

   Available Since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. MAC_C_INSR

   Plasmid

   #187772

   Purpose

   MAC-tagged gene expression of human INSR

   Depositor

   Markku Varjosalo

   Article

   Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

   Insert

   INSR (INSR Human)
   

   Expression

   Mammalian

   Available Since

   Aug. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. lenti-SAM-hygro

    Plasmid

    #138955

    Purpose

    Lentiviral vector for co-expressing spCas9, dRNA-MS2, p65 and HSF1 fused to MS2 binding protein, and hygromycin resistance gene

    Depositor

    Nadya Dimitrova

    Article

    Olivero et al Mol Cell. 2020 Feb 20;77(4):761-774.e8.

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6 AND EF1a

    Available Since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSBbi 41BBL-CD86 mIL-15

    Plasmid

    #183245

    Purpose

    Expression of human 41BBL, CD86, and membrane bound human IL-15 (IL-15-IL-15Ra) in a Sleeping Beauty transposon vector for use in artificial antigen presenting cells

    Depositor

    Alexander McLellan

    Article

    Dobson et al Immunol Cell Biol. 2023 Aug 16. doi: 10.

    Insert

    41BBL, CD86, mbIL-15 (IL-15-IL-15Ra)
    

    Expression

    Mammalian

    Available Since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCI-T7Max-UTR1-deGFP-8xHis-T500

    Plasmid

    #178422

    Purpose

    Expression of deGFP via a T7 Promoter in TXTL

    Depositor

    Kate Adamala

    Article

    Deich et al J Biol Eng. 2023 Jan 23;17(1):4. doi: 10

    Insert

    deGFP
    

    Use

    Synthetic Biology

    Tags

    8xHis Tag

    Expression

    Bacterial

    Promoter

    T7Max

    Available Since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSB1C3-J23110-B0034-mRFP1_Yellow

    Plasmid

    #160443

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Yellow chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

    Insert

    promoter, RBS, mRFP1E_Yellow
    

    Use

    Synthetic Biology

    Mutation

    BioBrick sites removed

    Available Since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBTK622

    Plasmid

    #180592

    Purpose

    tdk/kanR dual selection cassette for engineering the genome of Acinetobacter baylyi ADP1

    Depositor

    Jeffrey Barrick

    Article

    Suarez et al Nucleic Acids Res. 2020 May 7;48(8):4585

    Inserts

    tdk
    kanR
    

    Use

    Synthetic Biology

    Available Since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLVX-Tight-Puro_Nkx6.2

    Plasmid

    #64846

    Purpose

    A lentiviral vector for the expression of mouse Nkx6.2 under doxycyline control

    Depositor

    Paul Tesar

    Article

    Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

    Insert

    Nkx6.2 (Nkx6-2 Mouse)
    

    Use

    Lentiviral

    Expression

    Bacterial and Mammalian

    Available Since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNCS Citrine

    Plasmid

    #91761

    Purpose

    Bacterial expression of a fluorescent protein, Citrine

    Depositor

    Roger Tsien, Erik Rodriguez

    Article

    Griesbeck et al J Biol Chem. 2001 Aug 3;276(31):29188-94

    Insert

    Citrine
    

    Tags

    Hexa-Histidine tag, Xpress epitope for detection …

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTwist GFP-Linker-VDAC1

    Plasmid

    #211735

    Purpose

    Expresses GFP tagged VDAC1 in mammalian cells. GFP is attached to the N-terminus of VDAC1 with a flexible linker.

    Depositor

    Roland Malli

    Article

    Pilic et al bioRxiv 2023.09.08.556841

    Insert

    Voltage-dependent anion channel 1 (VDAC1 )
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. EGFP-Rab7A Q67L

    Plasmid

    #28049

    Depositor

    Qing Zhong

    Article

    Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

    Insert

    Rab7A (RAB7A Human)
    

    Tags

    2xHA and EGFP

    Expression

    Mammalian

    Mutation

    Q67L

    Available Since

    May 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV-CAG-hChR2-H134R-tdTomato

    Plasmid

    #28017

    Purpose

    AAV-mediated expression of hChR2(H134R) for neuronal excitation.

    Depositor

    Karel Svoboda

    Article

    Mao et al Neuron. 2011 Oct 6;72(1):111-23.

    Has Service

    AAV Retrograde

    Insert

    humanized ChR2-H134R-tdTomato
    

    Use

    AAV; Adeno associate viral

    Tags

    tdTomato

    Mutation

    H134R

    Promoter

    CAG

    Available Since

    Nov. 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pD649-HAsp-EDA-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156768

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    EDA (EDA Human)
    

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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