161,488 results

1. pOpen-SP6rpol

   Plasmid

   #165516

   Purpose

   DNA-dependent RNA polymerase derived from SP6 bacteriophage that catalyzes the transcription of viral genes.

   Depositor

   Drew Endy, FreeGenes Project, Jennifer Molloy

   Insert

   SP6 RNA Polymerase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 29, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pGDP4 ermC

   Plasmid

   #112884

   Purpose

   This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Plac promoter.

   Depositor

   Gerard Wright

   Article

   Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

   Insert

   ermC (ermM Staphylococcus epidermis)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Plac

   Available since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR223_SUV39H1_WT

   Plasmid

   #82236

   Purpose

   Gateway Donor vector containing SUV39H1 , part of the Target Accelerator Plasmid Collection.

   Depositor

   William Hahn, Jesse Boehm, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   SUV39H1 (SUV39H1 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   WT

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pXJ40 GADD34 (514-674aa)

   Plasmid

   #75480

   Purpose

   mammalian expression of GADD34 514-674 aa

   Depositor

   Shirish Shenolikar

   Article

   Choy et al Cell Rep. 2015 Jun 30;11(12):1885-91. do

   Insert

   GADD34 (PPP1R15A Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   expresses aa 514-674

   Promoter

   CMV

   Available since

   June 21, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pAG34

   Plasmid

   #35125

   Depositor

   John McCusker

   Article

   Goldstein et al Yeast. 1999 Oct;15(14):1541-53.

   Insert

   hphMX4
   

   Use

   Yeast replicating

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGDP3 arr

   Plasmid

   #112890

   Purpose

   This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

   Depositor

   Gerard Wright

   Article

   Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

   Insert

   arr
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Pbla

   Available since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGR595

   Plasmid

   #213785

   Purpose

   Integrates at the CAN1 locus the BadBoy2 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette.

   Depositor

   Chang Liu

   Insert

   BadBoy2 TP-DNAP1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. mPlum-PCNA-19-SV40NLS-4

   Plasmid

   #55994

   Purpose

   Localization: Nucleus, Excitation: 590, Emission: 649

   Depositor

   Michael Davidson

   Insert

   PCNA-19 SV40NLS (PCNA Human)
   

   Use

   Tags

   mPlum

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

9. GALT-SEP-mRuby3

   Plasmid

   #200938

   Purpose

   trans-Golgi localized pH sensor using sensorsuperecliptic pHluorin (SEP) and mRuby3

   Depositor

   Nir Hacohen

   Article

   Liu et al Science. 2023 Aug 4;381(6657):508-514. d

   Inserts

   GalT (B4GALT1 Human)
   SEP-mRuby3
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pXJ40 GADD34 (1-513aa)

    Plasmid

    #75479

    Purpose

    mammalian expression of GADD34 1-513 aa

    Depositor

    Shirish Shenolikar

    Article

    Choy et al Cell Rep. 2015 Jun 30;11(12):1885-91. do

    Insert

    GADD34 (PPP1R15A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    expresses aa 1-513

    Promoter

    CMV

    Available since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pFA6a- mEGFP-kanMX6

    Plasmid

    #105146

    Purpose

    Yeast gene targeting

    Depositor

    Jian-Qiu Wu, Thomas Pollard

    Article

    Wu et al Dev Cell. 2003 Nov;5(5):723-34.

    Insert

    mEGFP-kanMX6
    

    Use

    Tags

    mEGFP

    Expression

    Bacterial and Yeast

    Mutation

    A206K

    Promoter

    Available since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJ2072.2_c1

    Plasmid

    #140675

    Purpose

    CRISPRlator control (“open ring”)

    Depositor

    Yolanda Schaerli

    Article

    Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

    Insert

    CRISPRlator control (“open ring”)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSN23

    Plasmid

    #100108

    Purpose

    expresses a chimeric SOX2-activator fusion

    Depositor

    Mark Ptashne

    Article

    Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

    Insert

    mCitrine-P2A-SOX2 fused to a 'medium' VP16 domain (SOX2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGDP3 cat

    Plasmid

    #112888

    Purpose

    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

    Depositor

    Gerard Wright

    Article

    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

    Insert

    cat (Brevibacillus brevis) (cat Brevibacillus brevis)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Pbla

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGDP1 fosA

    Plasmid

    #112889

    Purpose

    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

    Depositor

    Gerard Wright

    Article

    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

    Insert

    fosA
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Pbla

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pX601-GFP

    Plasmid

    #84040

    Purpose

    Staphylococcus aureus (SaCas9) conjugated with GFP

    Depositor

    Yuet Wai Kan

    Article

    Ye et al Proc Natl Acad Sci U S A. 2016 Sep 20;11

    Insert

    SaCas9
    

    Use

    AAV and CRISPR

    Tags

    NLS and T2A-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. CROP_C9_Puro_AAVS1B

    Plasmid

    #183338

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets the AAVS1 locus

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    AAVS1B
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pENTR-MXC

    Plasmid

    #37140

    Depositor

    Robert Duronio

    Article

    White et al J Cell Biol. 2011 May 16;193(4):677-94.

    Insert

    Multi Sex combs (mxc Fly)
    

    Use

    Gateway

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

19. ERroGFP_iE_C204Q_pCDNA3

    Plasmid

    #48629

    Purpose

    Mammalian expression vector encoding for the ER targeted and FLAGM1 tagged insensitive variant of fluorescent redox probe roGFPiE with Cysteine 204 to Glutamine mutation (roGFP C147, E147b, C204Q)

    Depositor

    David Ron

    Article

    Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.

    Insert

    ERroGFP
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    insertion E147B, C204Q

    Promoter

    Available since

    Oct. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRAU168

    Plasmid

    #86834

    Purpose

    bacterial expression of anti-CRISPR protein AcrIIA1

    Depositor

    Joseph Bondy-Denomy

    Article

    Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

    Insert

    acrIIA1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge