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(Plasmid #110274)


Item Catalog # Description Quantity Price (USD)
Plasmid 110274 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy


  • Gene/Insert name
    Elongin BC
  • Alt name
    co-expresses Elongin B and Elongin C
  • Species
    H. sapiens (human)
  • Mutation
    Elongin B is full length. Elongin C contains a.a. 17-112.
  • Entrez Gene
    ELOC (a.k.a. SIII, TCEB1)
  • Entrez Gene
    ELOB (a.k.a. SIII, TCEB2)
  • Promoter T7

Cloning Information

  • Cloning method Unknown
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer pDuetDOWN-1 (5'-TTGTACACGGCCGCATAATC-3') or pDuetUP2 (5'-GATTATGCGGCCGTGTACAA-3')
  • 3′ sequencing primer T7-term
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Full length Elongin B was cloned into MCS1 NcoI site of pACYC-Duet1.

Elongin C (a.a. 17-112) was cloned in 2nd MCS with stop codon before any 3’ vector S tag. Therefore, these inserts produce untagged proteins.

Note: ATG from NdeI site precedes internal start Met17 of Elongin C giving start sequence MMYVK...


How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ELONGIN BC (XX01TCEB1A-c001) was a gift from Nicola Burgess-Brown (Addgene plasmid # 110274 ; ; RRID:Addgene_110274)