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(Plasmid #34885)


Item Catalog # Description Quantity Price (USD)
Plasmid 34885 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy


  • Gene/Insert name
    Sindbis virus envelope protein
  • Species
    Sindbus Virus
  • Insert Size (bp)
  • Mutation
    SGN UTR in E1 domain
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site None (unknown if destroyed)
  • 3′ cloning site None (unknown if destroyed)
  • 5′ sequencing primer pCMV-VSV-G-Fwd: AACCGGGCCCCTCTGCTAAC
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

2.2 expresses the mutated version of the Sinbis virus envelope protein (also referred to as m168), in addition to the SGN UTR mutations (AK226-227SG in E1 domain). This mutant reduces residual tropism while maintaining a high titer on targeted cells.

This plasmid was created using Addgene Plasmid pCMV-VSV-G (#8454), where the VSV-G was removed and replaced with the Sindbis virus envelope protein (above).

See map for construction details and recommended restriction digest analysis.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    2.2 was a gift from Irvin Chen (Addgene plasmid # 34885 ; ; RRID:Addgene_34885)
  • For your References section:

    A novel dual-targeted lentiviral vector leads to specific transduction of prostate cancer bone metastases in vivo after systemic administration. Pariente N, Morizono K, Virk MS, Petrigliano FA, Reiter RE, Lieberman JR, Chen IS. Mol Ther. 2007 Nov;15(11):1973-81. Epub 2007 Jul 24. 10.1038/ PubMed 17653099