159,238 results

1. Mito-TurboID

   Plasmid

   #200960

   Purpose

   Transiently expresses mitochondria localized TurboID with V5 tags

   Depositor

   Keriann Backus

   Article

   Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

   Insert

   mito-TurboID
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. KillerRed-LacI-NLS

   Plasmid

   #215102

   Purpose

   Expresses KillerRed-LacI-NLS in mammalian cells.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   KillerRed-LacI-NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn-JEDI-1P-Kv-WPRE

   Plasmid

   #202612

   Purpose

   Soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector for neuron-specific expression using the promoter hSyn

   Depositor

   Francois St-Pierre

   Article

   Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

   Insert

   JEDI-1P-Kv
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   June 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR223-IRAK4

   Plasmid

   #23749

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   IRAK4 (IRAK4 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

5. pUC19/mChe-Px

   Plasmid

   #183170

   Purpose

   Construct for transient expression of peroxisomes-targeted mCherry in plants.

   Depositor

   Yao-Guang Liu, Qinlong Zhu

   Article

   Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

   Insert

   mCherry-Px
   

   Use

   Tags

   10 aa peroxisome targeting signal 1 (IHHPRELSRL) …

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. PEmax-mRNA

   Plasmid

   #204472

   Purpose

   plasmid for PEmax mRNA IVT

   Depositor

   Scot Wolfe

   Article

   Ponnienselvan et al Nucleic Acids Res. 2023 May 29:gkad456.

   Insert

   Prime editor
   

   Use

   CRISPR

   Tags

   no

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   Aug. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. BE4-Gam

   Plasmid

   #100806

   Purpose

   Expresses BE4-Gam in mammalian cells

   Depositor

   David Liu

   Article

   Komor et al Sci Adv. 2017 Aug 30;3(8):eaao4774. doi:

   Insert

   BE4-Gam
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. phage UbiC NLS HA stdPCP stdGFP

   Plasmid

   #104099

   Purpose

   Lentiviral vector expressing synonymous tandem PP7 coat protein fused to synonymous tandem GFP. Used to label reporter mRNAs.

   Depositor

   Jeffrey Chao

   Article

   Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

   Insert

   stdPCP-stdGFP
   

   Use

   Lentiviral

   Tags

   N-terminal NLS, Two synonymous copies of GFP (C-t…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLminP_Luc2P_RE29

   Plasmid

   #90371

   Purpose

   SREBF1 - Sterol gene reporter

   Depositor

   Ramnik Xavier

   Article

   O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

   Insert

   Firefly luciferase
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   SREBPb

   Available since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pQE30-His-NDK

    Plasmid

    #124136

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Nucleotide diphosphate kinase (ndk Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. TFORF0172

    Plasmid

    #142551

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ONECUT2 (ONECUT2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA4/TO mTRPM7

    Plasmid

    #45482

    Depositor

    Andrew Scharenberg

    Article

    Nadler et al Nature. 2001 May 31;411(6837):590-5.

    Insert

    TRPM7 (Trpm7 Mouse)
    

    Use

    Tetracycline inducible

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Tetracycline-controlled CMV

    Available since

    May 21, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pCMV-NbALFA-degFusionRed

    Plasmid

    #199466

    Purpose

    Expression of the photodegradable protein degFusionRed fused to an anti-ALFA-tag-specific nanobody in mammalian cells.

    Depositor

    Vladislav Verkhusha

    Article

    Chernov et al Int J Mol Sci. 2023 Mar 30;24(7):6526. d

    Insert

    NbALFA-degFusionRed
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET29b-rhMOG

    Plasmid

    #225484

    Purpose

    Express the soluble domain of Myelin Oligodendrocyte Glycoprotein (rhMOG) in SHuffle E. coli

    Depositor

    John Pak

    Article

    Wu et al J Immunol Methods. 2024 Oct 13:113764. d

    Insert

    Soluble Domain of Myelin Oligodendrocyte Glycoprotein (MOG Human)
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-29 (signal peptide) and 150…

    Promoter

    T7

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDONR223-KHK

    Plasmid

    #23586

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    KHK (KHK Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pGL3 3 kb prom. CD274

    Plasmid

    #107004

    Purpose

    Modified Luciferase expression vector

    Depositor

    Julian Downward

    Article

    Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

    Insert

    CD274 (CD274 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUC19/Pd-mChe

    Plasmid

    #183172

    Purpose

    Construct for transient expression of plasmodesmata-targeted mCherry in plants.

    Depositor

    Yao-Guang Liu, Qinlong Zhu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Insert

    Pd-mCherry
    

    Use

    Tags

    AtPDCB1, A. thaliana PLASMODESMATA CALLOSE-BINDIN…

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. β3-integrin-YFP

    Plasmid

    #26653

    Depositor

    Jonathan Jones

    Article

    Tsuruta et al FASEB J. 2002 Jun . 16(8):866-8.

    Insert

    β3-integrin (ITGB3 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TFORF3383

    Plasmid

    #144859

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NFKBID (NFKBID Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJOG1368

    Plasmid

    #167301

    Purpose

    Level 0 module (CDS1) for further assemblies based on the Modular Cloning system. FCY-UPP gene conferring sensitivity to 5-Fluorocytosine

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Insert

    FCY-UPP fusion gene
    

    Use

    Synthetic Biology; Moclo level 0 vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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