pCDNA3 Flag MKK4(glu)
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14813||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5446
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)1151
Entrez GeneMAP2K4 (a.k.a. JNKK, JNKK1, MAPKK4, MEK4, MKK4, PRKMK4, SAPKK-1, SAPKK1, SEK1, SERK1, SKK1)
/ Fusion Protein
- Flag (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site Bam HI (not destroyed)
- 3′ cloning site Xba I (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCDNA3 Flag MKK4(glu) was a gift from Roger Davis (Addgene plasmid # 14813 ; http://n2t.net/addgene:14813 ; RRID:Addgene_14813)
For your References section:Integration of MAP kinase signal transduction pathways at the serum response element. Whitmarsh AJ, Shore P, Sharrocks AD, Davis RJ. Science. 1995 Jul 21. 269(5222):403-7. 10.1126/science.7618106 PubMed 7618106