|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||20745||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepLenti CMV/TO Puro DEST (670-1)
Backbone manufacturerEric Campeau (Addgene plasmid 17293)
- Backbone size w/o insert (bp) 9594
Vector typeMammalian Expression, Lentiviral ; Destination vector
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)2934
MutationK599A, dominant negative kinase mutant
Entrez GeneERN1 (a.k.a. IRE1, IRE1P, IRE1a, hIRE1p)
- Cloning method Gateway Cloning
- 5′ cloning site Gateway (unknown if destroyed)
- 3′ cloning site Gateway (unknown if destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer MSCV-rev (Common Sequencing Primers)
IRE1a cDNA was cut out of pENTR1A with EcoRI and XhoI and ligated into pLenti CMV/TO Puro DEST through Gateway cloning.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:hIRE1a K599A was a gift from Fumihiko Urano (Addgene plasmid # 20745 ; http://n2t.net/addgene:20745 ; RRID:Addgene_20745)
For your References section:The role of IRE1alpha in the degradation of insulin mRNA in pancreatic beta-cells. Lipson KL, Ghosh R, Urano F. PLoS ONE. 2008 . 3(2):e1648. 10.1371/journal.pone.0001648 PubMed 18286202