|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||16263||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5000
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
SpeciesH. sapiens (human)
Mutationisoleucine to glycine mutation at residue 642
Entrez GeneERN1 (a.k.a. IRE1, IRE1P, IRE1a, hIRE1p)
- Cloning method Restriction Enzyme
- 5′ sequencing primer N/A (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byRandy Kaufman lab created original pED-hIRE1 vector (see Tirasophon et al., Genes Dev 1998).
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
I642G mutation of pED-hIRE1 plasmid
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pED-hIRE1(I642G) was a gift from Peter Walter (Addgene plasmid # 16263 ; http://n2t.net/addgene:16263 ; RRID:Addgene_16263)
For your References section:IRE1 signaling affects cell fate during the unfolded protein response. Lin JH, Li H, Yasumura D, Cohen HR, Zhang C, Panning B, Shokat KM, Lavail MM, Walter P. Science. 2007 Nov 9. 318(5852):944-9. 10.1126/science.1146361 PubMed 17991856