Engineered CRISPR-Cas9 nuclease with expanded targeting space.
Nishimasu H, Shi X, Ishiguro S, Gao L, Hirano S, Okazaki S, Noda T, Abudayyeh OO, Gootenberg JS, Mori H, Oura S, Holmes B, Tanaka M, Seki M, Hirano H, Aburatani H, Ishitani R, Ikawa M, Yachie N, Zhang F, Nureki O
Science. 2018 Aug 30. pii: science.aas9129. doi: 10.1126/science.aas9129.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 117919 | pX330-SpCas9-NG | Expresses 3xFLAG-NLS-SpCas9-NG-NLS in mammalian cells. |
| 119861 | pSI-Target-AID-NG | Expresses Target-AID-NG (NLS-nSpCas9-NG-NLS-SH3-3xFLAG-PmCDA1-NLS-UGI) in mammalian cells. |