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(Plasmid #32704)


Item Catalog # Description Quantity Price (USD)
Plasmid 32704 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Richard O. Hynes group
  • Modifications to backbone
    1) GFP to dsRed 2) Thy1.1-mir30 to emGFP-miR (Invitrogen)
  • Vector type
    Mammalian Expression, Retroviral, RNAi, Cre/Lox
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    The depositor notes that XL1-Blue is also ok for general purpose use.
  • Copy number


  • Gene/Insert name
  • gRNA/shRNA sequence
    It is a vector for cloning new miRNA.

Cloning Information

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMSCV-FLIP-puro-dsRed-GFP-miRNA was a gift from Hans Clevers (Addgene plasmid # 32704 ; ; RRID:Addgene_32704)
  • For your References section:

    Controlled gene expression in primary Lgr5 organoid cultures. Koo BK, Stange DE, Sato T, Karthaus W, Farin HF, Huch M, van Es JH, Clevers H. Nat Methods. 2011 Dec 4. doi: 10.1038/nmeth.1802. 10.1038/nmeth.1802 PubMed 22138822