Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26610||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5400
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesR. norvegicus (rat)
Insert Size (bp)1500
Entrez GeneRps6kb1 (a.k.a. p70 S6K-alpha)
/ Fusion Protein
- Myc (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA-Myc S6K1 was a gift from Jie Chen (Addgene plasmid # 26610 ; http://n2t.net/addgene:26610 ; RRID:Addgene_26610)
For your References section:Phosphatidic acid-mediated mitogenic activation of mTOR signaling. Fang Y, Vilella-Bach M, Bachmann R, Flanigan A, Chen J. Science. 2001 Nov 30. 294(5548):1942-5. 10.1126/science.1066015 PubMed 11729323