pcDNA FLAG mTOR wt
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||22998||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5000
Vector typeMammalian Expression
Growth in Bacteria
SpeciesH. sapiens (human)
Entrez GeneMTOR (a.k.a. FRAP, FRAP1, FRAP2, RAFT1, RAPT1, SKS)
/ Fusion Protein
- FLAG (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (unknown if destroyed)
- 3′ cloning site KpnI* (unknown if destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
*Please note that there is also a KpnI site within the insert. If you plan to sub-clone, a straightforward cut and paste with KpnI may be difficult.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA FLAG mTOR wt was a gift from Jing Huang (Addgene plasmid # 22998)
For your References section:Target identification using drug affinity responsive target stability (DARTS). Lomenick B, Hao R, Jonai N, Chin RM, Aghajan M, Warburton S, Wang J, Wu RP, Gomez F, Loo JA, Wohlschlegel JA, Vondriska TM, Pelletier J, Herschman HR, Clardy J, Clarke CF, Huang J. Proc Natl Acad Sci U S A. 2009 Dec 7. ():. 10.1073/pnas.0910040106 PubMed 19995983