pcDNA FLAG mTOR wt
(Plasmid #22998)

• Depositing Lab: Jing Huang
• Publication: Lomenick et al Proc Natl Acad Sci U S A. 2009 Dec 7. ():.

Backbone

• Vector backbone: pcDNA3.1
• Backbone size w/o insert (bp): 5000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: mTOR
• Species: H. sapiens (human)
• Entrez Gene: MTOR (a.k.a. FRAP, FRAP1, FRAP2, RAFT1, RAPT1, SKS)
• Tag / Fusion Protein:
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (unknown if destroyed)
• 3′ cloning site: KpnI* (unknown if destroyed)
• 5′ sequencing primer: T7

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

*Please note that there is also a KpnI site within the insert. If you plan to sub-clone, a straightforward cut and paste with KpnI may be difficult.