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pSG5-ABL
(Plasmid #31284)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 31284 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pSG5
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 4076
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    V-abl Abelson murine leukemia viral oncogene homolog 1
  • Alt name
    ABL1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4500
  • GenBank ID
    NM_007313
  • Entrez Gene
    ABL1 (a.k.a. ABL, BCR-ABL, CHDSKM, JTK7, bcr/abl, c-ABL, c-ABL1, p150, v-abl)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SV40-pA-R
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The construct was made by Ron de Jong in 1993/1994. It contains the entire coding region of the Abl 1B variant (which has a putative N-terminal myristoylation site) but with the 5’ and 3’ untranslated regions truncated. This cDNA clone contains a P829L mutation in Abl 1B compared with reference sequence NM_007313

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSG5-ABL was a gift from Nora Heisterkamp (Addgene plasmid # 31284 ; http://n2t.net/addgene:31284 ; RRID:Addgene_31284)
  • For your References section:

    Bcr/Abl P190 interaction with Spa-1, a GTPase activating protein for the small GTPase Rap1. Yi SJ, Lee HT, Groffen J, Heisterkamp N. Int J Mol Med. 2008 Oct . 22(4):453-8. PubMed 18813851