pSG5-ABL
(Plasmid #31284)

• Depositing Lab: Nora Heisterkamp
• Publication: Yi et al Int J Mol Med. 2008 Oct . 22(4):453-8.

Backbone

• Vector backbone: pSG5
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 4076
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: V-abl Abelson murine leukemia viral oncogene homolog 1
• Alt name: ABL1
• Species: H. sapiens (human)
• Insert Size (bp): 4500
• GenBank ID: NM_007313
• Entrez Gene: ABL1 (a.k.a. ABL, BCR-ABL, CHDSKM, JTK7, bcr/abl, c-ABL, c-ABL1, p150, v-abl)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SV40-pA-R

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The construct was made by Ron de Jong in 1993/1994. It contains the entire coding region of the Abl 1B variant (which has a putative N-terminal myristoylation site) but with the 5’ and 3’ untranslated regions truncated. This cDNA clone contains a P829L mutation in Abl 1B compared with reference sequence NM_007313