|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||38159||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 7400
- Total vector size (bp) 14000
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameBCR/ABL P190
SpeciesH. sapiens (human)
Insert Size (bp)7000
MutationT117M in c-ABL1
Entrez GeneABL1 (a.k.a. ABL, BCR-ABL, CHDSKM, JTK7, bcr/abl, c-ABL, c-ABL1, p150, v-abl)
Entrez GeneBCR (a.k.a. ALL, BCR1, CML, D22S11, D22S662, PHL)
- Promoter SV40
/ Fusion Protein
- GST (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer GST or T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Constructed by Suparna Mishra. Generated in a 3-way ligation consisting of 5' 0.75 kb BamHI/Sal I + 3' 6.3 kb Sal I/EcoRI BCR/ABL fragments into pLEF digested with BamHIxEcoRI. The ABL part of the insert contains the natural TAG and 3' UT sequences.
Addgene NGS identified a sequence discrepancy at nucleotide 3168 resulting in a T117M mutation in the ABL-1 sequence, relative to Genbank ID NP_005148.2 and Addgene Plasmid #38158. This is not a known polymorphism and the effect of this, if any, on the protein is not known.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Bcr/Abl P190-pLEF was a gift from Nora Heisterkamp (Addgene plasmid # 38159 ; http://n2t.net/addgene:38159 ; RRID:Addgene_38159)
For your References section:Activity of the Bcr GTPase-activating domain is regulated through direct protein/protein interaction with the Rho guanine nucleotide dissociation inhibitor. Kweon SM, Cho YJ, Minoo P, Groffen J, Heisterkamp N. J Biol Chem. 2008 Feb 8;283(6):3023-30. Epub 2007 Dec 10. 10.1074/jbc.M705513200 PubMed 18070886