|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||31285||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4076
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer t7
- 3′ sequencing primer SV40-pA-R (Common Sequencing Primers)
This construct encodes a human chimeric P190-encoding Bcr/Abl oncogene.
The construct was made by Leena Haataja in 1993.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSG5-P190 was a gift from Nora Heisterkamp (Addgene plasmid # 31285 ; http://n2t.net/addgene:31285 ; RRID:Addgene_31285)
For your References section:Bcr/Abl P190 interaction with Spa-1, a GTPase activating protein for the small GTPase Rap1. Yi SJ, Lee HT, Groffen J, Heisterkamp N. Int J Mol Med. 2008 Oct . 22(4):453-8. PubMed 18813851