phage UbiC tagRFP-T-DDX6
(Plasmid #119947)

• Purpose: Lentiviral vector expressing tagRFP-T-DDX6 fusion protein
• Depositing Lab: Jeffrey Chao
• Publication: Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31036-0. doi: 10.1016/j.molcel.2018.12.006.

Backbone

• Vector backbone: pHAGE-UbiC
• Backbone size w/o insert (bp): 7000
• Total vector size (bp): 8456
• Vector type: Mammalian Expression, Bacterial Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: DDX6
• Species: H. sapiens (human)
• Insert Size (bp): 1446
• Entrez Gene: DDX6 (a.k.a. HLR2, IDDILF, P54, RCK, Rck/p54)
• Promoter: UbiC
• Tag / Fusion Protein:
  • tagRFP-T (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: GTGTGGCACAGCTAGTTCC

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/332502v1 for BioRxiv preprint