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Single-Molecule Imaging of mRNA Localization and Regulation during the Integrated Stress Response.
Wilbertz JH, Voigt F, Horvathova I, Roth G, Zhan Y, Chao JA
Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31036-0. doi: 10.1016/j.molcel.2018.12.006.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
119945pSF4 TetCMV intron 20xGCN4 Renilla FKBP Stop 24xMS2v5 SV40 CTE polyAExpresses SunTag-Renilla mRNA reporter in mammalian cells; note plasmid contains 24xGCN4 repeats
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119946pSF4 TetCMV 5'TOP intron 20xGCN4 Renilla FKBP Stop 24xMS2v5 SV40 CTE polyAExpresses 5'TOP-SunTag-Renilla mRNA reporter in mammalian cells; note plasmid contains 24xGCN4 repeats
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119947phage UbiC tagRFP-T-DDX6Lentiviral vector expressing tagRFP-T-DDX6 fusion protein
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119948phage UbiC SNAP-DDX6Lentiviral vector expressing SNAP-DDX6 fusion protein
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119949phage UbiC G3BP1-SNAPLentiviral vector expressing G3BP1-SNAP fusion protein
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119950phage UbiC G3BP1-GFP-GFPLentiviral vector expressing G3BP1-GFP fusion protein
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Antibodies from Article