pSF4 TetCMV 5'TOP intron 20xGCN4 Renilla FKBP Stop 24xMS2v5 SV40 CTE polyA
(Plasmid
#119946)
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PurposeExpresses 5'TOP-SunTag-Renilla mRNA reporter in mammalian cells; note plasmid contains 24xGCN4 repeats
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Depositing Lab
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Depositing OrganizationFriedrich Miescher Institute for Biomedical Research
Located in Switzerland -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 119946 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 119946-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 119946-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepSF4
- Backbone size w/o insert (bp) 10500
- Total vector size (bp) 11304
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Vector typeMammalian Expression, Bacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRenilla luciferase
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SpeciesRenilla reniformis
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Insert Size (bp)930
- Promoter TetCMV
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Tags
/ Fusion Proteins
- 5'TOP sequence
- 24xGCN4 repeats (SunTag) (N terminal on insert)
- FKBP (C terminal on insert)
- 24xMS2v5 stem loops in 3'UTR
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer AATACGACTCACTATAGGC
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/332502v1 for BioRxiv preprint
Note plasmid contains 24xGCN4 repeats and not 20xGCN4 as described in the name.
DNA (Catalog # 119946-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 119946-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSF4 TetCMV 5'TOP intron 20xGCN4 Renilla FKBP Stop 24xMS2v5 SV40 CTE polyA was a gift from Jeffrey Chao (Addgene plasmid # 119946 ; http://n2t.net/addgene:119946 ; RRID:Addgene_119946) -
For your References section:
Single-Molecule Imaging of mRNA Localization and Regulation during the Integrated Stress Response. Wilbertz JH, Voigt F, Horvathova I, Roth G, Zhan Y, Chao JA. Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31036-0. doi: 10.1016/j.molcel.2018.12.006. 10.1016/j.molcel.2018.12.006 PubMed 30661979
